DOI: 10.1073/pnas.69.5.1192. Low Activity of β-Galactosidase in Frameshift Mutants of <i>Escherichia coli</i>

Low Activity of β-Galactosidase in Frameshift Mutants of Escherichia coli

10.1073/pnas.69.5.1192

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

16 lac frameshift mutants induced by an acridine derivative, ICR-191D, in E. coli are leaky for β-galactosidase activity. Activities of all mutants differ from each other and from the wild type in their stability to thermal denaturation. The leakiness is under ribosomal control, since it is strongly reduced by strA restrictive mutations and is restored by ram mutations that reverse restriction. Addition of streptomycin during growth has an effect similar to the presence of the ram mutation. These ribosomal alterations do not modify the thermal stability of the enzyme. It is suggested that the leakiness is due to an infrequent 2- or 4-base reading close to the frameshift mutation site. The possibility that not only the ribosome, but also the reading context in the messenger, plays a role in securing code fidelity is discussed.

Bibliography

Atkins, J. F., Elseviers, D., & Gorini, L. (1972). Low Activity of Î²-Galactosidase in Frameshift Mutants of Escherichia coli. Proceedings of the National Academy of Sciences, 69(5), 1192â1195.

Authors 3

John F. Atkins (first)
Dirk Elseviers (additional)
Luigi Gorini (additional)

References 0 Referenced 78

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:34 a.m.)
Indexed	4 months, 2 weeks ago (April 8, 2025, 9:58 p.m.)
Issued	53 years, 3 months ago (May 1, 1972)
Published	53 years, 3 months ago (May 1, 1972)
Published Online	53 years, 3 months ago (May 1, 1972)
Published Print	53 years, 3 months ago (May 1, 1972)

Funders 0

None

BibTeX

@article{Atkins_1972, title={Low Activity of β-Galactosidase in Frameshift Mutants of Escherichia coli}, volume={69}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.69.5.1192}, DOI={10.1073/pnas.69.5.1192}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Atkins, John F. and Elseviers, Dirk and Gorini, Luigi}, year={1972}, month=may, pages={1192–1195} }

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