DOI: 10.1073/pnas.69.2.452. A New Synthetic RNA-Dependent DNA Polymerase from Human Tissue Culture Cells

A New Synthetic RNA-Dependent DNA Polymerase from Human Tissue Culture Cells

10.1073/pnas.69.2.452

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Two DNA polymerases that can copy synthetic RNA polymers are present in human tissue culture cells. These enzymes which have each been purified about 500-fold, are present in both HeLa cells, which are derived from a cervical carcinoma, and in WI-38 cells, a normal diploid strain originating from human embryonic lung tissue. These synthetic RNA-dependent DNA polymerases are identified by their ability to copy efficiently the ribo strand of synthetic oligonucleotide-homopolymer complexes, and differ in this respect from the known DNA-dependent DNA polymerases found in HeLa cells. The template requirements of these new DNA polymerases resemble that of the RNA-dependent DNA polymerases of the RNA tumor-viruses.

Bibliography

Fridlender, B., Fry, M., Bolden, A., & Weissbach, A. (1972). A New Synthetic RNA-Dependent DNA Polymerase from Human Tissue Culture Cells. Proceedings of the National Academy of Sciences, 69(2), 452â455.

Authors 4

Bertold Fridlender (first)
Michael Fry (additional)
Arthur Bolden (additional)
Arthur Weissbach (additional)

References 0 Referenced 100

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:32 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:28 a.m.)
Indexed	11 months, 1 week ago (Sept. 11, 2024, 10:55 a.m.)
Issued	53 years, 6 months ago (Feb. 1, 1972)
Published	53 years, 6 months ago (Feb. 1, 1972)
Published Online	53 years, 6 months ago (Feb. 1, 1972)
Published Print	53 years, 6 months ago (Feb. 1, 1972)

Funders 0

None

BibTeX

@article{Fridlender_1972, title={A New Synthetic RNA-Dependent DNA Polymerase from Human Tissue Culture Cells}, volume={69}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.69.2.452}, DOI={10.1073/pnas.69.2.452}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Fridlender, Bertold and Fry, Michael and Bolden, Arthur and Weissbach, Arthur}, year={1972}, month=feb, pages={452–455} }

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