DOI: 10.1073/pnas.69.12.3665. Translation of Encephalomyocarditis Viral RNA in Oocytes of <i>Xenopus laevis</i>

Translation of Encephalomyocarditis Viral RNA in Oocytes of Xenopus laevis

10.1073/pnas.69.12.3665

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

RNA from encephalomyocarditis virus was injected into oocytes of Xenopus laevis . After incubation of the oocytes in [ 35 S]methionine, polyacrylamide gel electrophoresis showed that a new series of polypeptides had been synthesized. They were identical in size to the polypeptides that appeared in ascites cells after infection with this virus. Electrophoretic and chromatographic analysis of the methionine-containing tryptic peptides from three of the induced polypeptides confirmed that they were virus-specific. All of the bands that appeared in ascites cells after infection also appeared in oocytes after injection of RNA from the virus. We conclude that Xenopus oocytes translate a mammalian viral mRNA faithfully and extensively, and perform normal post-translational modifications.

Bibliography

Laskey, R. A., Gurdon, J. B., & Crawford, L. V. (1972). Translation of Encephalomyocarditis Viral RNA in Oocytes of Xenopus laevis. Proceedings of the National Academy of Sciences, 69(12), 3665â3669.

Authors 3

R. A. Laskey (first)
J. B. Gurdon (additional)
L. V. Crawford (additional)

References 0 Referenced 35

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:33 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:23 a.m.)
Indexed	1 month, 4 weeks ago (June 26, 2025, 4:25 p.m.)
Issued	52 years, 8 months ago (Dec. 1, 1972)
Published	52 years, 8 months ago (Dec. 1, 1972)
Published Online	52 years, 8 months ago (Dec. 1, 1972)
Published Print	52 years, 8 months ago (Dec. 1, 1972)

Funders 0

None

BibTeX

@article{Laskey_1972, title={Translation of Encephalomyocarditis Viral RNA in Oocytes of Xenopus laevis}, volume={69}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.69.12.3665}, DOI={10.1073/pnas.69.12.3665}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Laskey, R. A. and Gurdon, J. B. and Crawford, L. V.}, year={1972}, month=dec, pages={3665–3669} }

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