DOI: 10.1073/pnas.68.9.2268. Purification and <i>In Vitro</i> Transcription of a Transfer RNA Gene

Purification and In Vitro Transcription of a Transfer RNA Gene

10.1073/pnas.68.9.2268

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A gene specifying tyrosine transfer RNA has been purified and transcribed in vitro . The purification procedure made use of two specialized transducing phages carrying the tRNA Tyr gene of Escherichia coli inserted into their DNA in opposite orientations. The separated heavy strands of the two phages were annealed and the single-stranded tails of the resulting hybrid were removed by digestion with Neurospora endonuclease. The size of the purified double-stranded structures was determined by electron microscopy. These isolated duplexes served as template for the in vitro transcription of tRNA Tyr -like molecules.

Bibliography

Daniel, V., Beckmann, J. S., Sarid, S., Grimberg, J. I., Herzberg, M., & Littauer, U. Z. (1971). Purification and In Vitro Transcription of a Transfer RNA Gene. Proceedings of the National Academy of Sciences, 68(9), 2268â2272.

Authors 6

Violet Daniel (first)
Jacques S. Beckmann (additional)
Sara Sarid (additional)
Jacob I. Grimberg (additional)
Max Herzberg (additional)
Uriel Z. Littauer (additional)

References 0 Referenced 8

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:30 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:32 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 3:23 p.m.)
Issued	54 years ago (Sept. 1, 1971)
Published	54 years ago (Sept. 1, 1971)
Published Online	54 years ago (Sept. 1, 1971)
Published Print	54 years ago (Sept. 1, 1971)

Funders 0

None

BibTeX

@article{Daniel_1971, title={Purification and In Vitro Transcription of a Transfer RNA Gene}, volume={68}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.68.9.2268}, DOI={10.1073/pnas.68.9.2268}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Daniel, Violet and Beckmann, Jacques S. and Sarid, Sara and Grimberg, Jacob I. and Herzberg, Max and Littauer, Uriel Z.}, year={1971}, month=sep, pages={2268–2272} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T20:23:13Z",
    "timestamp": 1700770993850
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "9",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1971,
        9
      ]
    ]
  },
  "abstract": "<jats:p>\n            A gene specifying tyrosine transfer RNA has been purified and transcribed\n            <jats:italic>in vitro</jats:italic>\n            . The purification procedure made use of two specialized transducing phages carrying the tRNA\n            <jats:sup>Tyr</jats:sup>\n            gene of\n            <jats:italic>Escherichia coli</jats:italic>\n            inserted into their DNA in opposite orientations. The separated heavy strands of the two phages were annealed and the single-stranded tails of the resulting hybrid were removed by digestion with\n            <jats:italic>Neurospora</jats:italic>\n            endonuclease. The size of the purified double-stranded structures was determined by electron microscopy. These isolated duplexes served as template for the\n            <jats:italic>in vitro</jats:italic>\n            transcription of tRNA\n            <jats:sup>Tyr</jats:sup>\n            -like molecules.\n          </jats:p>",
  "DOI": "10.1073/pnas.68.9.2268",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T06:30:31Z",
    "timestamp": 1149057031000
  },
  "page": "2268-2272",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 8,
  "title": "Purification and\n            <i>In Vitro</i>\n            Transcription of a Transfer RNA Gene",
  "prefix": "10.1073",
  "volume": "68",
  "author": [
    {
      "given": "Violet",
      "family": "Daniel",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Biochemistry, The Weizmann Institute of Science, Rehovot, Israel"
        }
      ]
    },
    {
      "given": "Jacques S.",
      "family": "Beckmann",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, The Weizmann Institute of Science, Rehovot, Israel"
        }
      ]
    },
    {
      "given": "Sara",
      "family": "Sarid",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel"
        }
      ]
    },
    {
      "given": "Jacob I.",
      "family": "Grimberg",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, The Weizmann Institute of Science, Rehovot, Israel"
        }
      ]
    },
    {
      "given": "Max",
      "family": "Herzberg",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biological Ultrastructure, The Weizmann Institute of Science, Rehovot, Israel"
        }
      ]
    },
    {
      "given": "Uriel Z.",
      "family": "Littauer",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biochemistry, The Weizmann Institute of Science, Rehovot, Israel"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1971,
        9
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.68.9.2268",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T14:32:01Z",
    "timestamp": 1649860321000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.68.9.2268"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1971,
        9
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "9",
    "published-print": {
      "date-parts": [
        [
          1971,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.68.9.2268"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.68.9.2268",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1971,
        9
      ]
    ]
  },
  "assertion": [
    {
      "value": "1971-09-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}