DOI: 10.1073/pnas.68.9.2195. DNA-Directed Cell-Free Synthesis of Biologically Active Transfer RNA: su + III Tyrosyl-tRNA

DNA-Directed Cell-Free Synthesis of Biologically Active Transfer RNA: su + III Tyrosyl-tRNA

10.1073/pnas.68.9.2195

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Biologically active su + III tyrosyl-tRNA has been synthesized in a DNA-directed cell-free system from Escerichia coli . Such a system should be most useful for studying the mechanism of tRNA synthesis. This tRNA is capable of suppressing amber mutations in the gene coding for β-galactosidase (EC 3.2.1.23) and, therefore, must be capable of being charged and transferring amino acids. A 4-fold stimulation in the activity of the tRNA formed de novo is obtained with isopentenyl pyrophosphate, a compound involved in the post-transcriptional acylation of an adenine base adjacent to the anticodon. It has been suggested elsewhere that formation of RNA subject to stringent control may be inhibited by guanosine tetraphosphate (ppGpp). However, guanosine tetraphosphate did not affect the synthesis of su + III tyrosyl-tRNA, even though the synthesis of this tRNA is subject to stringent control.

Bibliography

Zubay, G., Cheong, L., & Gefter, M. (1971). DNA-Directed Cell-Free Synthesis of Biologically Active Transfer RNA: su + III Tyrosyl-tRNA. Proceedings of the National Academy of Sciences, 68(9), 2195â2197.

Authors 3

Geoffrey Zubay (first)
Loretta Cheong (additional)
Malcolm Gefter (additional)

References 0 Referenced 24

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:30 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:31 a.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 4:20 p.m.)
Issued	53 years, 11 months ago (Sept. 1, 1971)
Published	53 years, 11 months ago (Sept. 1, 1971)
Published Online	53 years, 11 months ago (Sept. 1, 1971)
Published Print	53 years, 11 months ago (Sept. 1, 1971)

Funders 0

None

BibTeX

@article{Zubay_1971, title={DNA-Directed Cell-Free Synthesis of Biologically Active Transfer RNA: su + III Tyrosyl-tRNA}, volume={68}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.68.9.2195}, DOI={10.1073/pnas.68.9.2195}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Zubay, Geoffrey and Cheong, Loretta and Gefter, Malcolm}, year={1971}, month=sep, pages={2195–2197} }

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