DOI: 10.1073/pnas.68.7.1512. Acetyl CoA Carboxylase: Isolation and Characterization of Native Biotin Carboxyl Carrier Protein

Acetyl CoA Carboxylase: Isolation and Characterization of Native Biotin Carboxyl Carrier Protein

10.1073/pnas.68.7.1512

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A large form of biotin carboxyl carrier protein (BCCP L ) has been isolated from extracts of Escherichia coli . It has a minimal molecular weight of 20,000, according to its behavior on sodium dodecylsulfate-polyacrylamide gel electrophoresis, and contains approximately 1 mol of biotin per 22,000 g of protein. BCCP L exhibits K m values, in the biotin carboxylase and transcarboxylase half-reactions of acetyl CoA carboxylase, of 2 × 10 -7 M and 4 × 10 -7 M, respectively; these values are 50-100 times lower than those obtained with smaller forms of BCCP previously isolated. Electrophoresis of crude extracts of E. coli indicates that the major biotin-containing protein migrates at the same rate as BCCP L , which suggests that BCCP L is the native form of BCCP in E. coli .

Bibliography

Fall, R. R., Nervi, A. M., Alberts, A. W., & Vagelos, P. R. (1971). Acetyl CoA Carboxylase: Isolation and Characterization of Native Biotin Carboxyl Carrier Protein. Proceedings of the National Academy of Sciences, 68(7), 1512â1515.

Authors 4

R. Ray Fall (first)
A. M. Nervi (additional)
Alfred W. Alberts (additional)
P. Roy Vagelos (additional)

References 0 Referenced 31

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:29 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:42 a.m.)
Indexed	1 month ago (July 30, 2025, 10:37 a.m.)
Issued	54 years, 1 month ago (July 1, 1971)
Published	54 years, 1 month ago (July 1, 1971)
Published Online	54 years, 1 month ago (July 1, 1971)
Published Print	54 years, 1 month ago (July 1, 1971)

Funders 0

None

BibTeX

@article{Fall_1971, title={Acetyl CoA Carboxylase: Isolation and Characterization of Native Biotin Carboxyl Carrier Protein}, volume={68}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.68.7.1512}, DOI={10.1073/pnas.68.7.1512}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Fall, R. Ray and Nervi, A. M. and Alberts, Alfred W. and Vagelos, P. Roy}, year={1971}, month=jul, pages={1512–1515} }

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