DOI: 10.1073/pnas.68.4.841. High-Resolution X-Ray Diffraction Patterns of Crystalline Transfer RNA That Show Helical Regions

High-Resolution X-Ray Diffraction Patterns of Crystalline Transfer RNA That Show Helical Regions

10.1073/pnas.68.4.841

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Yeast phenylalanyl transfer RNA crystallizes in a simple orthorhombic unit cell ( a = 33.2, b = 56.1, c = 161 Å), and the crystal yields an x-ray diffraction pattern with a resolution of 2.3 Å. From an analysis of the packing in the unit cell it is concluded that the molecular dimensions are approximately 80 by 33 by 28 Å. The diffraction pattern viewed along the a -axis has a distribution characteristic of double-helical nucleic acids. However, this distribution is not found when the pattern is viewed along the b -axis. This has been interpreted as indicating that the double-helical portions of the transfer RNA molecule are approximately half a helical turn in length, and therefore can contain 4-7 base pairs. These results are consistent with the cloverleaf formulation of transfer RNA secondary structure.

Bibliography

Kim, S. H., Quigley, G., Suddath, F. L., & Rich, A. (1971). High-Resolution X-Ray Diffraction Patterns of Crystalline Transfer RNA That Show Helical Regions. Proceedings of the National Academy of Sciences, 68(4), 841â845.

Authors 4

Sung Hou Kim (first)
Gary Quigley (additional)
F. L. Suddath (additional)
Alexander Rich (additional)

References 0 Referenced 48

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:28 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:23 a.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 3:31 a.m.)
Issued	54 years, 5 months ago (April 1, 1971)
Published	54 years, 5 months ago (April 1, 1971)
Published Online	54 years, 5 months ago (April 1, 1971)
Published Print	54 years, 5 months ago (April 1, 1971)

Funders 0

None

BibTeX

@article{Kim_1971, title={High-Resolution X-Ray Diffraction Patterns of Crystalline Transfer RNA That Show Helical Regions}, volume={68}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.68.4.841}, DOI={10.1073/pnas.68.4.841}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kim, Sung Hou and Quigley, Gary and Suddath, F. L. and Rich, Alexander}, year={1971}, month=apr, pages={841–845} }

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