DOI: 10.1073/pnas.68.4.832. Release, Identification, and Isolation of Messenger RNA from Mammalian Ribosomes

Release, Identification, and Isolation of Messenger RNA from Mammalian Ribosomes

10.1073/pnas.68.4.832

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The puromycin-induced dissociation, at high ionic strength, of active ribosomes from reticulocytes resulted in the release of protein-free, apparently undegraded, messenger RNA for globin, which was identified by centrifugation on a sucrose density gradient. This procedure should make messenger RNA from various cells available for isolation on a large scale, and has several advantages over procedures that use detergents or magnesium chelators.

Bibliography

Blobel, G. (1971). Release, Identification, and Isolation of Messenger RNA from Mammalian Ribosomes. Proceedings of the National Academy of Sciences, 68(4), 832â835.

Authors 1

Günter Blobel (first)

References 0 Referenced 48

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:28 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:23 a.m.)
Indexed	9 months, 1 week ago (Nov. 19, 2024, 10:59 a.m.)
Issued	54 years, 4 months ago (April 1, 1971)
Published	54 years, 4 months ago (April 1, 1971)
Published Online	54 years, 4 months ago (April 1, 1971)
Published Print	54 years, 4 months ago (April 1, 1971)

Funders 0

None

BibTeX

@article{Blobel_1971, title={Release, Identification, and Isolation of Messenger RNA from Mammalian Ribosomes}, volume={68}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.68.4.832}, DOI={10.1073/pnas.68.4.832}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Blobel, Günter}, year={1971}, month=apr, pages={832–835} }

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