DOI: 10.1073/pnas.68.4.795. Isolation of Transducing Particles of ϕ80 Bacteriophage That Carry Different Regions of the <i>Escherichia coli</i> Genome

Isolation of Transducing Particles of ϕ80 Bacteriophage That Carry Different Regions of the Escherichia coli Genome

10.1073/pnas.68.4.795

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

It has been possible to mate two strains harboring F-prime (F′) factors and to isolate from such matings rare recombinants that behave as though the two episomes had fused. Thus, two genes not previously linked may be brought into close proximity. An F′ factor carrying the attachment site for ϕ80 was fused with one carrying the met-ppc-arg region of the chromosome. Lysogenization of such a strain, followed by induction, led to the isolation of ϕ80 arg + and ϕ80 met + transducing phages. This technique may be utilized as a general method for joining diverse bacterial genes to the genome of phage ϕ80.

Bibliography

Press, R., Glansdorff, N., Miner, P., Vries, J. D., Kadner, R., & Maas, W. K. (1971). Isolation of Transducing Particles of Ï80 Bacteriophage That Carry Different Regions of the Escherichia coli Genome. Proceedings of the National Academy of Sciences, 68(4), 795â798.

Authors 6

R. Press (first)
N. Glansdorff (additional)
P. Miner (additional)
J. De Vries (additional)
R. Kadner (additional)
W. K. Maas (additional)

References 0 Referenced 55

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:28 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:22 a.m.)
Indexed	1 year, 7 months ago (Jan. 18, 2024, 12:28 a.m.)
Issued	54 years, 5 months ago (April 1, 1971)
Published	54 years, 5 months ago (April 1, 1971)
Published Online	54 years, 5 months ago (April 1, 1971)
Published Print	54 years, 5 months ago (April 1, 1971)

Funders 0

None

BibTeX

@article{Press_1971, title={Isolation of Transducing Particles of ϕ80 Bacteriophage That Carry Different Regions of the Escherichia coli Genome}, volume={68}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.68.4.795}, DOI={10.1073/pnas.68.4.795}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Press, R. and Glansdorff, N. and Miner, P. and Vries, J. De and Kadner, R. and Maas, W. K.}, year={1971}, month=apr, pages={795–798} }

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