DOI: 10.1073/pnas.68.4.761. Purification and DNA Synthesis in Cell-Free Extracts: Properties of DNA Polymerase II

Purification and DNA Synthesis in Cell-Free Extracts: Properties of DNA Polymerase II

10.1073/pnas.68.4.761

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The major DNA-synthesizing enzyme present in Pol A 1 - Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg ++ , NH 4 + , and native DNA for maximal activity. The enzyme activity is sensitive to sulfhydryl reagents and is insensitive to anti-DNA polymerase I antiserum. A second DNA-synthesizing enzyme, present in low amounts, has been identified in Pol A 1 - extracts. The relationship of this enzyme to DNA polymerases I and II is discussed.

Bibliography

Kornberg, T., & Gefter, M. L. (1971). Purification and DNA Synthesis in Cell-Free Extracts: Properties of DNA Polymerase II. Proceedings of the National Academy of Sciences, 68(4), 761â764.

Authors 2

Thomas Kornberg (first)
Malcolm L. Gefter (additional)

References 0 Referenced 146

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:28 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:23 a.m.)
Indexed	1 year ago (Aug. 3, 2024, 10:27 p.m.)
Issued	54 years, 4 months ago (April 1, 1971)
Published	54 years, 4 months ago (April 1, 1971)
Published Online	54 years, 4 months ago (April 1, 1971)
Published Print	54 years, 4 months ago (April 1, 1971)

Funders 0

None

BibTeX

@article{Kornberg_1971, title={Purification and DNA Synthesis in Cell-Free Extracts: Properties of DNA Polymerase II}, volume={68}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.68.4.761}, DOI={10.1073/pnas.68.4.761}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kornberg, Thomas and Gefter, Malcolm L.}, year={1971}, month=apr, pages={761–764} }

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