DOI: 10.1073/pnas.68.12.3223. Mechanism of Action of Bacitracin: Complexation with Metal Ion and C <sub>55</sub> -Isoprenyl Pyrophosphate

Mechanism of Action of Bacitracin: Complexation with Metal Ion and C 55 -Isoprenyl Pyrophosphate

10.1073/pnas.68.12.3223

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The inhibition by bacitracin of the enzymatic dephosphorylation of C 55 -isoprenyl pyrophosphate is abolished by the addition of chelating agents. If, however, the chelating agent is added after a preincubation of bacitracin with a divalent cation and the lipid substrate, then its addition has little effect, indicating that bacitracin, metal ion, and C 55 -isoprenyl pyrophosphate form a complex. Various divalent cations can participate in complex formation, but monovalent cations are ineffective. A direct demonstration of the formation of a complex between the C 55 -isoprenyl pyrophosphate and bacitracin in the presence of metal ions was obtained. Molecular models that show one possible conformation for a complex between bacitracin and the C 55 -isoprenyl pyrophosphate, in which the metal ion acts as a bridge between the two compounds, are presented.

Bibliography

Stone, K. J., & Strominger, J. L. (1971). Mechanism of Action of Bacitracin: Complexation with Metal Ion and C 55 -Isoprenyl Pyrophosphate. Proceedings of the National Academy of Sciences, 68(12), 3223â3227.

Authors 2

K. John Stone (first)
Jack L. Strominger (additional)

References 0 Referenced 310

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:27 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:35 a.m.)
Indexed	2 weeks, 1 day ago (Aug. 21, 2025, 2:16 p.m.)
Issued	53 years, 9 months ago (Dec. 1, 1971)
Published	53 years, 9 months ago (Dec. 1, 1971)
Published Online	53 years, 9 months ago (Dec. 1, 1971)
Published Print	53 years, 9 months ago (Dec. 1, 1971)

Funders 0

None

BibTeX

@article{Stone_1971, title={Mechanism of Action of Bacitracin: Complexation with Metal Ion and C 55 -Isoprenyl Pyrophosphate}, volume={68}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.68.12.3223}, DOI={10.1073/pnas.68.12.3223}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Stone, K. John and Strominger, Jack L.}, year={1971}, month=dec, pages={3223–3227} }

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