Abstract
A bacterial restriction endonuclease has been used to produce specific fragments of SV40 DNA. Digestion of DNA from plaque-purified stocks of SV40 with the restriction endonuclease from Hemophilus influenzae gave 11 fragments resolvable by polyacrylamide gel electrophoresis, eight of which were equimolar with the original DNA. The fragments ranged from about 6.5 × 10 5 to 7.4 × 10 4 daltons, as determined by electron microscopy, DNA content, or electrophoretic mobility.
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Dates
Type | When |
---|---|
Created | 19 years, 2 months ago (May 31, 2006, 2:27 a.m.) |
Deposited | 3 years, 4 months ago (April 13, 2022, 10:35 a.m.) |
Indexed | 5 days, 15 hours ago (Aug. 24, 2025, 7 p.m.) |
Issued | 53 years, 8 months ago (Dec. 1, 1971) |
Published | 53 years, 8 months ago (Dec. 1, 1971) |
Published Online | 14 years, 5 months ago (March 30, 2011) |
Published Print | 53 years, 8 months ago (Dec. 1, 1971) |
@article{Danna_1971, title={Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae}, volume={68}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.68.12.2913}, DOI={10.1073/pnas.68.12.2913}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Danna, Kathleen and Nathans, Daniel}, year={1971}, month=dec, pages={2913–2917} }