DOI: 10.1073/pnas.67.2.537. Peptide Chain Termination: Effect of Protein S on Ribosomal Binding of Release Factors

Peptide Chain Termination: Effect of Protein S on Ribosomal Binding of Release Factors

10.1073/pnas.67.2.537

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The protein factor S, previously shown to stimulate polypeptide chain termination in bacterial extracts, has two effects upon the complex formed between ribosomes, release factor, and terminator (trinucleotide) codon: (1) in the absence of GTP or GDP, S stimulates formation of an [R·UAA·ribosome] intermediate, and (2) in the presence of GTP or GDP, S participates in dissociation of this intermediate. Factor S can stimulate fMet release from [fMet-tRNA f ·AUG·ribosome] intermediates in either the presence or absence of GTP or GDP. A model is proposed which relates the in vitro effects of S ± GTP (or GDP) on fMet release to the effects of S ± GTP (or GDP) on the binding and dissociation of R factor from ribosomes.

Bibliography

Goldstein, J. L., & Caskey, C. T. (1970). Peptide Chain Termination: Effect of Protein S on Ribosomal Binding of Release Factors. Proceedings of the National Academy of Sciences, 67(2), 537â543.

Authors 2

J. L. Goldstein (first)
C. T. Caskey (additional)

References 0 Referenced 35

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:23 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:43 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:46 p.m.)
Issued	54 years, 10 months ago (Oct. 1, 1970)
Published	54 years, 10 months ago (Oct. 1, 1970)
Published Online	54 years, 10 months ago (Oct. 1, 1970)
Published Print	54 years, 10 months ago (Oct. 1, 1970)

Funders 0

None

BibTeX

@article{Goldstein_1970, title={Peptide Chain Termination: Effect of Protein S on Ribosomal Binding of Release Factors}, volume={67}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.67.2.537}, DOI={10.1073/pnas.67.2.537}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Goldstein, J. L. and Caskey, C. T.}, year={1970}, month=oct, pages={537–543} }

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