DOI: 10.1073/pnas.67.1.305. A Protein Binding Assay for Adenosine 3′:5′-Cyclic Monophosphate

A Protein Binding Assay for Adenosine 3′:5′-Cyclic Monophosphate

10.1073/pnas.67.1.305

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A simple and sensitive assay for adenosine 3′:5′-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10 -9 M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP.

Bibliography

Gilman, A. G. (1970). A Protein Binding Assay for Adenosine 3â²:5â²-Cyclic Monophosphate. Proceedings of the National Academy of Sciences, 67(1), 305â312.

Authors 1

Alfred G. Gilman (first)

References 0 Referenced 2,000

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:22 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:34 a.m.)
Indexed	2 weeks, 6 days ago (Aug. 7, 2025, 4:57 p.m.)
Issued	54 years, 11 months ago (Sept. 1, 1970)
Published	54 years, 11 months ago (Sept. 1, 1970)
Published Online	54 years, 11 months ago (Sept. 1, 1970)
Published Print	54 years, 11 months ago (Sept. 1, 1970)

Funders 0

None

BibTeX

@article{Gilman_1970, title={A Protein Binding Assay for Adenosine 3′:5′-Cyclic Monophosphate}, volume={67}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.67.1.305}, DOI={10.1073/pnas.67.1.305}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Gilman, Alfred G.}, year={1970}, month=sep, pages={305–312} }

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