DOI: 10.1073/pnas.66.4.1260. <i>In Vitro</i> TRANSCRIPTION OF A TRANSFER RNA GENE

In Vitro TRANSCRIPTION OF A TRANSFER RNA GENE

10.1073/pnas.66.4.1260

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The Escherichia coli tRNA Tyr gene carried by the ϕ80psu 3 + transducing phage was transcribed in vitro by DNA-dependent RNA polymerase. The enzymatically synthesized tRNA Tyr -like polynucleotide chains were detected by competition with purified E. coli 32 P-tRNA Tyr for specific hybridization sites on ϕ80psu 3 + DNA. Analysis by sucrose gradient centrifugation showed the tRNA Tyr -like chains to possess a heterogeneous distribution with respect to sedimentation coefficients, with a broad peak around 8 S. The presence of the termination factor ρ during the transcription did not significantly reduce the average size of the in vitro synthesized tRNA Tyr -like chains.

Bibliography

Daniel, V., Sarid, S., Beckmann, J. S., & Littauer, U. Z. (1970). In Vitro TRANSCRIPTION OF A TRANSFER RNA GENE. Proceedings of the National Academy of Sciences, 66(4), 1260â1266.

Authors 4

Violet Daniel (first)
Sara Sarid (additional)
Jacques S. Beckmann (additional)
Uriel Z. Littauer (additional)

References 0 Referenced 19

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:21 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:26 a.m.)
Indexed	1 year, 1 month ago (Aug. 5, 2024, 4:39 p.m.)
Issued	55 years, 1 month ago (Aug. 1, 1970)
Published	55 years, 1 month ago (Aug. 1, 1970)
Published Online	55 years, 1 month ago (Aug. 1, 1970)
Published Print	55 years, 1 month ago (Aug. 1, 1970)

Funders 0

None

BibTeX

@article{Daniel_1970, title={In Vitro TRANSCRIPTION OF A TRANSFER RNA GENE}, volume={66}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.66.4.1260}, DOI={10.1073/pnas.66.4.1260}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Daniel, Violet and Sarid, Sara and Beckmann, Jacques S. and Littauer, Uriel Z.}, year={1970}, month=aug, pages={1260–1266} }

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