DOI: 10.1073/pnas.65.3.675. Specific Nucleolar and Nucleoplasmic RNA Polymerases

Specific Nucleolar and Nucleoplasmic RNA Polymerases

10.1073/pnas.65.3.675

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The DNA-dependent RNA polymerase activity present in rat liver nuclei has been solubilized and purified from whole nuclei and from subnuclear fractions. As reported earlier (Roeder, R. G., and W. J. Rutter, Nature , 224, 234 (1969)), two major chromatographically distinct enzymatic species (I and II) are present in whole nuclei. Subfractionation of whole nuclei into nucleolar and nucleoplasmic fractions had little effect on the total recovery of activity. Purified nucleoli contain predominantly polymerase I, whereas the nucleoplasmic fraction is greatly enriched for polymerase II. A third minor peak of activity has also been resolved in the nucleoplasmic preparations. We conclude that the RNA polymerases are specifically localized within the nucleus and may, therefore, play specific roles in the regulation of genetic transcription.

Bibliography

Roeder, R. G., & Rutter, W. J. (1970). Specific Nucleolar and Nucleoplasmic RNA Polymerases. Proceedings of the National Academy of Sciences, 65(3), 675â682.

Authors 2

Robert G. Roeder (first)
William J. Rutter (additional)

References 0 Referenced 284

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:19 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:22 a.m.)
Indexed	4 weeks ago (Aug. 5, 2025, 8:59 a.m.)
Issued	55 years, 6 months ago (March 1, 1970)
Published	55 years, 6 months ago (March 1, 1970)
Published Online	55 years, 6 months ago (March 1, 1970)
Published Print	55 years, 6 months ago (March 1, 1970)

Funders 0

None

BibTeX

@article{Roeder_1970, title={Specific Nucleolar and Nucleoplasmic RNA Polymerases}, volume={65}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.65.3.675}, DOI={10.1073/pnas.65.3.675}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Roeder, Robert G. and Rutter, William J.}, year={1970}, month=mar, pages={675–682} }

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