DOI: 10.1073/pnas.62.4.1203. TRANSFER RNA, III. RECONSTITUTION OF ALANINE ACCEPTOR ACTIVITY FROM FRAGMENTS PRODUCED BY SPECIFIC CLEAVAGE OF tRNA <sup>Ala</sup> <sub>II</sub> AT ITS ANTICODON

TRANSFER RNA, III. RECONSTITUTION OF ALANINE ACCEPTOR ACTIVITY FROM FRAGMENTS PRODUCED BY SPECIFIC CLEAVAGE OF tRNA Ala II AT ITS ANTICODON

10.1073/pnas.62.4.1203

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

This report describes experiments showing that tRNA Ala II can be cleaved specifically at the G residue of its anticodon to give “half” molecules in high yield. Neither of these fragments has alanine-acceptor activity, but this activity can be reconstituted by mixing the fragments in the presence of Mg +2 . In dilute salt, on the other hand, the active complex dissociates spontaneously at 25° into the two fragments. Thus, both “halves” of tRNA Ala II are necessary for acceptor activity and, in the presence of Mg +2 , combination of these fragments to give an active duplex is thermodynamically favorable.

Bibliography

Imura, N., Schwam, H., & Chambers, R. W. (1969). TRANSFER RNA, III. RECONSTITUTION OF ALANINE ACCEPTOR ACTIVITY FROM FRAGMENTS PRODUCED BY SPECIFIC CLEAVAGE OF tRNA Ala II AT ITS ANTICODON. Proceedings of the National Academy of Sciences, 62(4), 1203â1209.

Authors 3

Nobumasa Imura (first)
Harvey Schwam (additional)
Robert W. Chambers (additional)

References 0 Referenced 27

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 2:12 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10 a.m.)
Indexed	1 year, 7 months ago (Jan. 18, 2024, 9:37 p.m.)
Issued	56 years, 5 months ago (April 1, 1969)
Published	56 years, 5 months ago (April 1, 1969)
Published Online	56 years, 5 months ago (April 1, 1969)
Published Print	56 years, 5 months ago (April 1, 1969)

Funders 0

None

BibTeX

@article{Imura_1969, title={TRANSFER RNA, III. RECONSTITUTION OF ALANINE ACCEPTOR ACTIVITY FROM FRAGMENTS PRODUCED BY SPECIFIC CLEAVAGE OF tRNA Ala II AT ITS ANTICODON}, volume={62}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.62.4.1203}, DOI={10.1073/pnas.62.4.1203}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Imura, Nobumasa and Schwam, Harvey and Chambers, Robert W.}, year={1969}, month=apr, pages={1203–1209} }

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