DOI: 10.1073/pnas.62.4.1159. SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN <i>Escherichia coli</i> : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM

SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM

10.1073/pnas.62.4.1159

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The 23 S twisted circular form of Col E 1 DNA has been isolated from Escherichia coli as a tightly associated DNA-protein complex with a sedimentation coefficient of approximately 24 S . Treatment of this complex with pronase, trypsin, sodium dodecyl sulfate, Sarkosyl, or heat results in a conversion to a slower sedimenting form of 17 S or 18 S , as determined by centrifugation in neutral sucrose gradients. These treatments do not alter the sedimentation properties of noncomplexes supercoiled Col E 1 DNA even in the presence of the Col E 1 -protein complex. Electron microscopic analyses indicate that the decrease in sedimentation rate of the Col E 1 -protein complex after treatment with these various agents is largely owing to an induced transition of Col E 1 DNA from the supercoiled to the open circular state.

Bibliography

Clewell, D. B., & Helinski, D. R. (1969). SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli â¯: PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM. Proceedings of the National Academy of Sciences, 62(4), 1159â1166.

Authors 2

Don B. Clewell (first)
Donald R. Helinski (additional)

References 0 Referenced 1,083

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 2:12 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 10:01 a.m.)
Indexed	3 weeks, 1 day ago (Aug. 7, 2025, 4:59 p.m.)
Issued	56 years, 4 months ago (April 1, 1969)
Published	56 years, 4 months ago (April 1, 1969)
Published Online	56 years, 4 months ago (April 1, 1969)
Published Print	56 years, 4 months ago (April 1, 1969)

Funders 0

None

BibTeX

@article{Clewell_1969, title={SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli  : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM}, volume={62}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.62.4.1159}, DOI={10.1073/pnas.62.4.1159}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Clewell, Don B. and Helinski, Donald R.}, year={1969}, month=apr, pages={1159–1166} }

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