Molecular mechanism of recruitment of TFIIF- associating RNA polymerase C-terminal domain phosphatase (FCP1) by transcription factor IIF
10.1073/pnas.262798199
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

After mRNA transcription termination in eukaryotes, the hyperphosphorylated form of RNA polymerase II (pol II0) must be recycled by TFIIF-associating C-terminal domain phosphatase (FCP1), the phosphatase responsible for dephosphorylating the C-terminal domain of the largest polymerase subunit. Transcription factor (TF)-IIF stimulates the activity of FCP1, and the RNA polymerase II-associating protein 74 subunit of TFIIF forms a complex with FCP1 in both human and yeast. Here, we report a cocrystal structure of the winged-helix domain of human RNA polymerase II-associating protein 74 bound to the α-helical C terminus of human FCP1 (residues 944–961). These results illustrate the molecular mechanism by which TFIIF efficiently recruits FCP1 to the pol II transcription machinery for recycling of the polymerase.

Bibliography

Kamada, K., Roeder, R. G., & Burley, S. K. (2003). Molecular mechanism of recruitment of TFIIF- associating RNA polymerase C-terminal domain phosphatase (FCP1) by transcription factor IIF. Proceedings of the National Academy of Sciences, 100(5), 2296–2299.

Authors 3
  1. Katsuhiko Kamada (first)
  2. Robert G. Roeder (additional)
  3. Stephen K. Burley (additional)
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Dates
Type When
Created 22 years, 5 months ago (March 4, 2003, 1:36 p.m.)
Deposited 3 years, 2 months ago (June 7, 2022, 2:08 a.m.)
Indexed 4 months, 2 weeks ago (April 13, 2025, 6:43 a.m.)
Issued 22 years, 6 months ago (Feb. 18, 2003)
Published 22 years, 6 months ago (Feb. 18, 2003)
Published Online 22 years, 6 months ago (Feb. 18, 2003)
Published Print 22 years, 5 months ago (March 4, 2003)
Funders 0

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@article{Kamada_2003, title={Molecular mechanism of recruitment of TFIIF- associating RNA polymerase C-terminal domain phosphatase (FCP1) by transcription factor IIF}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.262798199}, DOI={10.1073/pnas.262798199}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kamada, Katsuhiko and Roeder, Robert G. and Burley, Stephen K.}, year={2003}, month=feb, pages={2296–2299} }