DOI: 10.1073/pnas.261714999. DEC1 is a downstream target of TGF-β with sequence-specific transcriptional repressor activities

DEC1 is a downstream target of TGF-β with sequence-specific transcriptional repressor activities

10.1073/pnas.261714999

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

To identify genes that mediate transforming growth factor-β (TGF-β) signaling, a colorectal cancer cell line that was sensitive to the growth inhibitory effects of this cytokine was created. We then determined the global gene expression profiles of these cells, and those of HaCaT human keratinocytes, in the presence and absence of TGF-β. Of the several genes identified in this screen, DEC1 was of particular note in light of the rapidity and consistency of its induction and its potential biochemical activities. We identified a consensus DNA-binding site for DEC1 and showed that DEC1 could repress the transcription of a reporter containing this binding site in its promoter. Finally, both alleles of the DEC1 locus in HaCaT cells were inactivated through targeted homologous recombination. This approach revealed that DEC1 induction was not required for the growth inhibition mediated by TGF-β in this line. However, DEC1 may function in concert with other signaling components to mediate certain biologic effects of TGF-β.

Bibliography

Zawel, L., Yu, J., Torrance, C. J., Markowitz, S., Kinzler, K. W., Vogelstein, B., & Zhou, S. (2002). DEC1 is a downstream target of TGF-Î² with sequence-specific transcriptional repressor activities. Proceedings of the National Academy of Sciences, 99(5), 2848â2853.

Authors 7

Leigh Zawel (first)
Jian Yu (additional)
Christopher J. Torrance (additional)
Sanford Markowitz (additional)
Kenneth W. Kinzler (additional)
Bert Vogelstein (additional)
Shibin Zhou (additional)

References 43 Referenced 74

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:37 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 9:02 a.m.)
Indexed	4 months, 2 weeks ago (April 13, 2025, 9:04 a.m.)
Issued	23 years, 5 months ago (March 5, 2002)
Published	23 years, 5 months ago (March 5, 2002)
Published Online	23 years, 5 months ago (March 5, 2002)
Published Print	23 years, 5 months ago (March 5, 2002)

Funders 0

None

BibTeX

@article{Zawel_2002, title={DEC1 is a downstream target of TGF-β with sequence-specific transcriptional repressor activities}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.261714999}, DOI={10.1073/pnas.261714999}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Zawel, Leigh and Yu, Jian and Torrance, Christopher J. and Markowitz, Sanford and Kinzler, Kenneth W. and Vogelstein, Bert and Zhou, Shibin}, year={2002}, month=mar, pages={2848–2853} }

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