DOI: 10.1073/pnas.261408198. A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in <i>Drosophila</i>

A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila

10.1073/pnas.261408198

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In Drosophila , enhancer trap strategies allow rapid access to expression patterns, molecular data, and mutations in trapped genes. However, they do not give any information at the protein level, e.g., about the protein subcellular localization. Using the green fluorescent protein (GFP) as a mobile artificial exon carried by a transposable P-element, we have developed a protein trap system. We screened for individual flies, in which GFP tags full-length endogenous proteins expressed from their endogenous locus, allowing us to observe their cellular and subcellular distribution. GFP fusions are targeted to virtually any compartment of the cell. In the case of insertions in previously known genes, we observe that the subcellular localization of the fusion protein corresponds to the described distribution of the endogenous protein. The artificial GFP exon does not disturb upstream and downstream splicing events. Many insertions correspond to genes not predicted by the Drosophila Genome Project. Our results show the feasibility of a protein trap in Drosophila . GFP reveals in real time the dynamics of protein's distribution in the whole, live organism and provides useful markers for a number of cellular structures and compartments.

Bibliography

Morin, X., Daneman, R., Zavortink, M., & Chia, W. (2001). A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila. Proceedings of the National Academy of Sciences, 98(26), 15050â15055.

Authors 4

Xavier Morin (first)
Richard Daneman (additional)
Michael Zavortink (additional)
William Chia (additional)

References 32 Referenced 730

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:36 a.m.)
Deposited	3 years, 4 months ago (April 25, 2022, 8:40 p.m.)
Indexed	2 days, 16 hours ago (Aug. 26, 2025, 2:47 a.m.)
Issued	23 years, 8 months ago (Dec. 11, 2001)
Published	23 years, 8 months ago (Dec. 11, 2001)
Published Online	23 years, 8 months ago (Dec. 11, 2001)
Published Print	23 years, 8 months ago (Dec. 18, 2001)

Funders 0

None

BibTeX

@article{Morin_2001, title={A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.261408198}, DOI={10.1073/pnas.261408198}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Morin, Xavier and Daneman, Richard and Zavortink, Michael and Chia, William}, year={2001}, month=dec, pages={15050–15055} }

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