DOI: 10.1073/pnas.260489497. Structural organization of yeast and mammalian mediator complexes

Structural organization of yeast and mammalian mediator complexes

10.1073/pnas.260489497

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Structures of yeast Mediator complex, of a related complex from mouse cells and of thyroid hormone receptor-associated protein complex from human cells have been determined by three-dimensional reconstruction from electron micrographs of single particles. All three complexes show a division in two parts, a “head” domain and a combined “middle-tail” domain. The head domains of the three complexes appear most similar and interact most closely with RNA polymerase II. The middle-tail domains show the greatest structural divergence and, in the case of the tail domain, may not interact with polymerase at all. Consistent with this structural divergence, analysis of a yeast Mediator mutant localizes subunits that are not conserved between yeast and mammalian cells to the tail domain. Biochemically defined Rgr1 and Srb4 modules of yeast Mediator are then assigned to the middle and head domains.

Bibliography

Dotson, M. R., Yuan, C. X., Roeder, R. G., Myers, L. C., Gustafsson, C. M., Jiang, Y. W., Li, Y., Kornberg, R. D., & Asturias, F. J. (2000). Structural organization of yeast and mammalian mediator complexes. Proceedings of the National Academy of Sciences, 97(26), 14307â14310.

Authors 9

Michaela R. Dotson (first)
Chao Xing Yuan (additional)
Robert G. Roeder (additional)
Lawrence C. Myers (additional)
Claes M. Gustafsson (additional)
Yi Wei Jiang (additional)
Yang Li (additional)
Roger D. Kornberg (additional)
Francisco J. Asturias (additional)

References 27 Referenced 172

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:44 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 9:36 a.m.)
Indexed	3 weeks ago (Aug. 5, 2025, 8:28 a.m.)
Issued	24 years, 8 months ago (Dec. 12, 2000)
Published	24 years, 8 months ago (Dec. 12, 2000)
Published Online	24 years, 8 months ago (Dec. 12, 2000)
Published Print	24 years, 8 months ago (Dec. 19, 2000)

Funders 0

None

BibTeX

@article{Dotson_2000, title={Structural organization of yeast and mammalian mediator complexes}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.260489497}, DOI={10.1073/pnas.260489497}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Dotson, Michaela R. and Yuan, Chao Xing and Roeder, Robert G. and Myers, Lawrence C. and Gustafsson, Claes M. and Jiang, Yi Wei and Li, Yang and Kornberg, Roger D. and Asturias, Francisco J.}, year={2000}, month=dec, pages={14307–14310} }

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