DOI: 10.1073/pnas.251401598. The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum

The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum

10.1073/pnas.251401598

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

gp78, also known as the tumor autocrine motility factor receptor, is a transmembrane protein whose expression is correlated with tumor metastasis. We establish that gp78 is a RING finger-dependent ubiquitin protein ligase (E3) of the endoplasmic reticulum (ER). Consistent with this, gp78 specifically recruits MmUBC7, a ubiquitin-conjugating enzyme (E2) implicated in ER-associated degradation (ERAD), through a region distinct from the RING finger. gp78 can target itself for proteasomal degradation in a RING finger- and MmUBC7-dependent manner. Importantly, gp78 can also mediate degradation of CD3-δ, a well-characterized ERAD substrate. In contrast, gp78 lacking an intact RING finger or its multiple membrane-spanning domains stabilizes CD3-δ. gp78 has thus been found to be an example of a mammalian cellular E3 intrinsic to the ER, suggesting a potential link between ubiquitylation, ERAD, and metastasis.

Bibliography

Fang, S., Ferrone, M., Yang, C., Jensen, J. P., Tiwari, S., & Weissman, A. M. (2001). The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum. Proceedings of the National Academy of Sciences, 98(25), 14422â14427.

Authors 6

Shengyun Fang (first)
Marco Ferrone (additional)
Cuihong Yang (additional)
Jane P. Jensen (additional)
Swati Tiwari (additional)
Allan M. Weissman (additional)

References 48 Referenced 357

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 9:49 a.m.)
Indexed	2 days, 22 hours ago (Aug. 19, 2025, 6:22 a.m.)
Issued	23 years, 8 months ago (Nov. 27, 2001)
Published	23 years, 8 months ago (Nov. 27, 2001)
Published Online	23 years, 8 months ago (Nov. 27, 2001)
Published Print	23 years, 8 months ago (Dec. 4, 2001)

Funders 0

None

BibTeX

@article{Fang_2001, title={The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.251401598}, DOI={10.1073/pnas.251401598}, number={25}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Fang, Shengyun and Ferrone, Marco and Yang, Cuihong and Jensen, Jane P. and Tiwari, Swati and Weissman, Allan M.}, year={2001}, month=nov, pages={14422–14427} }

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