DOI: 10.1073/pnas.241365698. Pause and rotation of F <sub>1</sub> -ATPase during catalysis

Pause and rotation of F 1 -ATPase during catalysis

10.1073/pnas.241365698

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

F 1 -ATPase is a rotary motor enzyme in which a single ATP molecule drives a 120° rotation of the central γ subunit relative to the surrounding α 3 β 3 ring. Here, we show that the rotation of F 1 -ATPase spontaneously lapses into long (≈30 s) pauses during steady-state catalysis. The effects of ADP-Mg and mutation on the pauses, as well as kinetic comparison with bulk-phase catalysis, strongly indicate that the paused enzyme corresponds to the inactive state of F 1 -ATPase previously known as the ADP-Mg inhibited form in which F 1 -ATPase fails to release ADP-Mg from catalytic sites. The pausing position of the γ subunit deviates from the ATP-waiting position and is most likely the recently found intermediate 90° position.

Bibliography

Hirono-Hara, Y., Noji, H., Nishiura, M., Muneyuki, E., Hara, K. Y., Yasuda, R., Kinosita, K., & Yoshida, M. (2001). Pause and rotation of F 1 -ATPase during catalysis. Proceedings of the National Academy of Sciences, 98(24), 13649â13654.

Authors 8

Yoko Hirono-Hara (first)
Hiroyuki Noji (additional)
Masaya Nishiura (additional)
Eiro Muneyuki (additional)
Kiyotaka Y. Hara (additional)
Ryohei Yasuda (additional)
Kazuhiko Kinosita (additional)
Masasuke Yoshida (additional)

References 25 Referenced 170

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 9:08 a.m.)
Indexed	2 weeks, 5 days ago (Aug. 6, 2025, 9:21 a.m.)
Issued	23 years, 9 months ago (Nov. 13, 2001)
Published	23 years, 9 months ago (Nov. 13, 2001)
Published Online	23 years, 9 months ago (Nov. 13, 2001)
Published Print	23 years, 9 months ago (Nov. 20, 2001)

Funders 0

None

BibTeX

@article{Hirono_Hara_2001, title={Pause and rotation of F 1 -ATPase during catalysis}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.241365698}, DOI={10.1073/pnas.241365698}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hirono-Hara, Yoko and Noji, Hiroyuki and Nishiura, Masaya and Muneyuki, Eiro and Hara, Kiyotaka Y. and Yasuda, Ryohei and Kinosita, Kazuhiko and Yoshida, Masasuke}, year={2001}, month=nov, pages={13649–13654} }

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