DOI: 10.1073/pnas.240345197. Glucose depletion causes haploid invasive growth in yeast

Glucose depletion causes haploid invasive growth in yeast

10.1073/pnas.240345197

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Haploid yeast invades solid agar in response to nutrient limitation. To decipher the cues that underlie invasion, we have developed a single cell invasive growth assay. Using this assay, as well as the traditional plate-washing assay, we show that invasive growth occurs in response to glucose depletion. In the absence of glucose (or other fermentable sugar), individual cells adopted a nonaxial budding pattern and elongated morphology within the first cell divisions, and invasion into the agar was observed in microcolonies containing as few as 10 cells. In support of this observation, we found that glucose suppressed the hyperinvasive growth morphology of STE11 - 4, pbs2, hsl7 , and RAS2V19 mutations. In addition, removal of glucose from YPD medium caused constitutive invasion in wild-type cells. We tested glucose control proteins for a role in invasion and found that Snf1, a protein required for derepression of glucose-repressed genes, was required for invasive growth. The transcription factor Sip4, which interacts with Snf1 and is induced during the diauxic shift, had an inhibitory role on invasive growth, suggesting that multiple mechanisms are required for glucose depletion-dependent invasion.

Bibliography

Cullen, P. J., & Sprague, G. F. (2000). Glucose depletion causes haploid invasive growth in yeast. Proceedings of the National Academy of Sciences, 97(25), 13619â13624.

Authors 2

Paul J. Cullen (first)
George F. Sprague (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:44 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 9:28 a.m.)
Indexed	3 weeks, 1 day ago (Aug. 7, 2025, 5:20 p.m.)
Issued	24 years, 9 months ago (Nov. 28, 2000)
Published	24 years, 9 months ago (Nov. 28, 2000)
Published Online	24 years, 9 months ago (Nov. 28, 2000)
Published Print	24 years, 8 months ago (Dec. 5, 2000)

Funders 0

None

BibTeX

@article{Cullen_2000, title={Glucose depletion causes haploid invasive growth in yeast}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.240345197}, DOI={10.1073/pnas.240345197}, number={25}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Cullen, Paul J. and Sprague, George F.}, year={2000}, month=nov, pages={13619–13624} }

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