DOI: 10.1073/pnas.232442599. Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold

Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold

10.1073/pnas.232442599

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Isolated newt ( Notophthalmus viridescens ) chromosomes were studied by using micromechanical force measurement during nuclease digestion. Micrococcal nuclease and short-recognition-sequence blunt-cutting restriction enzymes first remove the native elastic response of, and then to go on to completely disintegrate, single metaphase newt chromosomes. These experiments rule out the possibility that the mitotic chromosome is based on a mechanically contiguous internal non-DNA (e.g., protein) “scaffold”; instead, the mechanical integrity of the metaphase chromosome is due to chromatin itself. Blunt-cutting restriction enzymes with longer recognition sequences only partially disassemble mitotic chromosomes and indicate that chromatin in metaphase chromosomes is constrained by isolated chromatin-crosslinking elements spaced by ≈15 kb.

Bibliography

Poirier, M. G., & Marko, J. F. (2002). Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold. Proceedings of the National Academy of Sciences, 99(24), 15393â15397.

Authors 2

Michael G. Poirier (first)
John F. Marko (additional)

References 29 Referenced 157

10.1146/annurev.biochem.69.1.115
10.1038/35087089
10.1016/0092-8674(77)90280-X
10.1016/0092-8674(79)90325-8
10.1101/SQB.1978.042.01.036
10.1016/S0022-2836(86)80010-9
10.1083/jcb.127.2.303
10.1016/0092-8674(88)90239-5
10.1016/0092-8674(94)90502-9
10.1016/S0959-437X(98)80005-1
10.1023/A:1016690802570
Lodish H. Baltimore D. Berk A. Zipursky S. L. Matsudaria P. & Darnell J. (1995) Molecular Cell Biology (Scientific American New York) pp. 349–353.
Wolffe A. P. (1995) Chromatin: Structure and Function (Academic New York) Sec. 2.4.1.
Lewin B. (2000) Genes VII (Oxford New York) pp. 551–552.
van Holde K. E. (1989) Chromatin (Springer New York) pp. 346–350. (10.1007/978-1-4612-3490-6)
Cole A. (1967) Theor. Exp. Biol.1,305-375. / Theor. Exp. Biol. by Cole A. (1967)
10.1083/jcb.139.1.1
10.1091/mbc.11.1.269
10.1083/jcb.97.2.542
10.1111/j.1432-0436.1976.tb01472.x
10.1083/jcb.145.2.215
10.1016/S0960-9822(01)00180-4
10.1091/mbc.01-08-0401
10.1002/jcb.10132
10.1103/PhysRevE.58.3347
10.1002/(SICI)1097-4644(199704)65:1<114::AID-JCB12>3.0.CO;2-K
10.1006/jmbi.1995.0606
10.1006/jmbi.2000.3531
10.1083/jcb.105.1.77

Dates

Type	When
Created	22 years, 9 months ago (Nov. 26, 2002, 12:42 p.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 9:30 a.m.)
Indexed	1 month, 2 weeks ago (July 20, 2025, 12:18 a.m.)
Issued	22 years, 9 months ago (Nov. 18, 2002)
Published	22 years, 9 months ago (Nov. 18, 2002)
Published Online	22 years, 9 months ago (Nov. 18, 2002)
Published Print	22 years, 9 months ago (Nov. 26, 2002)

Funders 0

None

BibTeX

@article{Poirier_2002, title={Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.232442599}, DOI={10.1073/pnas.232442599}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Poirier, Michael G. and Marko, John F.}, year={2002}, month=nov, pages={15393–15397} }

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