DOI: 10.1073/pnas.231473398. COMPASS: A complex of proteins associated with a trithorax-related SET domain protein

COMPASS: A complex of proteins associated with a trithorax-related SET domain protein

10.1073/pnas.231473398

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The trithorax genes encode an evolutionarily conserved family of proteins that function to maintain specific patterns of gene expression throughout cellular development. Members of this protein family contain a highly conserved 130- to 140-amino acid motif termed the SET domain. We report the purification and molecular identification of the subunits of a protein complex in the yeast Saccharomyces cerevisiae that includes the trithorax-related protein Set1. This protein complex, which we have named COMPASS (Complex Proteins Associated with Set1), consists of seven polypeptides ranging from 130 to 25 kDa. The same seven proteins were identified in COMPASS purified either by conventional biochemical chromatography or tandem-affinity tagging of the individual subunits of the complex. Null mutants missing any one of the six nonessential subunits of COMPASS grow more slowly than wild-type cells under normal conditions and demonstrate growth sensitivity to hydroxyurea. Furthermore, gene expression profiles of strains missing either of two nonessential subunits of COMPASS are altered in similar ways, suggesting these proteins have similar roles in gene expression in vivo . Molecular characterization of trithorax complexes will facilitate defining the role of this class of proteins in the regulation of gene expression and how their misregulation results in the development of human cancer.

Bibliography

Miller, T., Krogan, N. J., Dover, J., Erdjument-Bromage, H., Tempst, P., Johnston, M., Greenblatt, J. F., & Shilatifard, A. (2001). COMPASS: A complex of proteins associated with a trithorax-related SET domain protein. Proceedings of the National Academy of Sciences, 98(23), 12902â12907.

Authors 8

Trissa Miller (first)
Nevan J. Krogan (additional)
Jim Dover (additional)
H. Erdjument-Bromage (additional)
Paul Tempst (additional)
Mark Johnston (additional)
Jack F. Greenblatt (additional)
Ali Shilatifard (additional)

References 34 Referenced 518

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:32 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 9:42 a.m.)
Indexed	5 days, 1 hour ago (Aug. 20, 2025, 9:15 a.m.)
Issued	23 years, 9 months ago (Oct. 30, 2001)
Published	23 years, 9 months ago (Oct. 30, 2001)
Published Online	23 years, 9 months ago (Oct. 30, 2001)
Published Print	23 years, 9 months ago (Nov. 6, 2001)

Funders 0

None

BibTeX

@article{Miller_2001, title={COMPASS: A complex of proteins associated with a trithorax-related SET domain protein}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.231473398}, DOI={10.1073/pnas.231473398}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Miller, Trissa and Krogan, Nevan J. and Dover, Jim and Erdjument-Bromage, H. and Tempst, Paul and Johnston, Mark and Greenblatt, Jack F. and Shilatifard, Ali}, year={2001}, month=oct, pages={12902–12907} }

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