DOI: 10.1073/pnas.230262797. The transcriptional responses of respiratory epithelial cells to<i>Bordetella pertussis</i>reveal host defensive and pathogen counter-defensive strategies

The transcriptional responses of respiratory epithelial cells toBordetella pertussisreveal host defensive and pathogen counter-defensive strategies

10.1073/pnas.230262797

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Bordetella pertussis, the causative agent of whooping cough, has many well-studied virulence factors and a characteristic clinical presentation. Despite this information, it is not clear howB. pertussisinteraction with host cells leads to disease. In this study, we examined the interaction ofB. pertussiswith a human bronchial epithelial cell line (BEAS-2B) and measured host transcriptional profiles by using high-density DNA microarrays. The early transcriptional response to this pathogen is dominated by altered expression of cytokines, DNA-binding proteins, and NFκB-regulated genes. This previously unrecognized response toB. pertussiswas modified in similar but nonidentical fashions by the antiinflammatory agents dexamethasone and sodium salicylate. Cytokine protein expression was confirmed, as was neutrophil chemoattraction. We show thatB. pertussisinduces mucin gene transcription by BEAS-2B cells then counters this defense by using mucin as a binding substrate. A set of genes is described for which the catalytic activity of pertussis toxin is both necessary and sufficient to regulate transcription. Host genomic transcriptional profiling, in combination with functional assays to evaluate subsequent biological events, provides insight into the complex interaction of host and pathogen.

Bibliography

Belcher, C. E., Drenkow, J., Kehoe, B., Gingeras, T. R., McNamara, N., Lemjabbar, H., Basbaum, C., & Relman, D. A. (2000). The transcriptional responses of respiratory epithelial cells toBordetella pertussisreveal host defensive and pathogen counter-defensive strategies. Proceedings of the National Academy of Sciences, 97(25), 13847â13852.

Authors 8

Christopher E. Belcher (first)
Jörg Drenkow (additional)
Bettina Kehoe (additional)
Thomas R. Gingeras (additional)
Nancy McNamara (additional)
Hassan Lemjabbar (additional)
Carol Basbaum (additional)
David A. Relman (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:36 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:17 a.m.)
Indexed	2 days, 20 hours ago (Sept. 3, 2025, 6:45 a.m.)
Issued	24 years, 9 months ago (Nov. 21, 2000)
Published	24 years, 9 months ago (Nov. 21, 2000)
Published Online	24 years, 9 months ago (Nov. 21, 2000)
Published Print	24 years, 9 months ago (Dec. 5, 2000)

Funders 0

None

BibTeX

@article{Belcher_2000, title={The transcriptional responses of respiratory epithelial cells toBordetella pertussisreveal host defensive and pathogen counter-defensive strategies}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.230262797}, DOI={10.1073/pnas.230262797}, number={25}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Belcher, Christopher E. and Drenkow, Jörg and Kehoe, Bettina and Gingeras, Thomas R. and McNamara, Nancy and Lemjabbar, Hassan and Basbaum, Carol and Relman, David A.}, year={2000}, month=nov, pages={13847–13852} }

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  "URL": "http://dx.doi.org/10.1073/pnas.230262797",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        2000,
        11,
        21
      ]
    ]
  },
  "assertion": [
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      "order": 0,
      "name": "received",
      "label": "Received",
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        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2000-11-21",
      "order": 2,
      "name": "published",
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}