DOI: 10.1073/pnas.2235589100. A specific endoribonuclease, RNase P, affects gene expression of polycistronic operon mRNAs

A specific endoribonuclease, RNase P, affects gene expression of polycistronic operon mRNAs

10.1073/pnas.2235589100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The rnpA mutation, A49, in Escherichia coli reduces the level of RNase P at 43°C because of a temperature-sensitive mutation in C5 protein, the protein subunit of the enzyme. Microarray analysis reveals the expression of several noncoding intergenic regions that are increased at 43°C compared with 30°C. These regions are substrates for RNase P, and they are cleaved less efficiently than, for example, tRNA precursors. An analysis of the tna, secG, rbs , and his operons, all of which contain RNase P cleavage sites, indicates that RNase P affects gene expression for regions downstream of its cleavage sites.

Bibliography

Li, Y., & Altman, S. (2003). A specific endoribonuclease, RNase P, affects gene expression of polycistronic operon mRNAs. Proceedings of the National Academy of Sciences, 100(23), 13213â13218.

Authors 2

Yong Li (first)
Sidney Altman (additional)

References 27 Referenced 94

Zabin I. & Fowler A. V. (1978) in The Operon eds. Miller J. H. & Reznikoff W. S. (Cold Spring Harbor Lab. Press Plainview NY) pp. 111-112.
Beckwith J. (1996) in Escherichia coli and Salmonella: Cellular and Molecular Biology ed. Neidhardt F. C. (Am. Soc. Microbiol. Press Washington DC) p. 1230.
Altman S. & Kirsebom L. (1999) in The RNA World eds. Gesteland R. Cech T. R. & Atkins J. F. (Cold Spring Harbor Lab. Press Plainview NY) 2nd Ed. pp. 351-380.
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Schedl, P., Primakoff, P. & Roberts, J. (1974) in Brookhaven Symposia in Biology, ed. Dunn, J. J. (Brookhaven Natl. Lab., Brookhaven, NY), Vol. 26, pp. 31-76. / Brookhaven Symposia in Biology (1974)
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Dates

Type	When
Created	21 years, 9 months ago (Nov. 16, 2003, 3:42 p.m.)
Deposited	3 years, 4 months ago (April 25, 2022, 9:35 p.m.)
Indexed	3 months ago (May 27, 2025, 8:57 a.m.)
Issued	21 years, 9 months ago (Oct. 29, 2003)
Published	21 years, 9 months ago (Oct. 29, 2003)
Published Online	21 years, 9 months ago (Oct. 29, 2003)
Published Print	21 years, 9 months ago (Nov. 11, 2003)

Funders 0

None

BibTeX

@article{Li_2003, title={A specific endoribonuclease, RNase P, affects gene expression of polycistronic operon mRNAs}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.2235589100}, DOI={10.1073/pnas.2235589100}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Li, Yong and Altman, Sidney}, year={2003}, month=oct, pages={13213–13218} }

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