DOI: 10.1073/pnas.222371099. Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice

Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice

10.1073/pnas.222371099

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The kinesin superfamily proteins (KIFs) play essential roles in receptor transportation along the microtubules. KIF17 transports the N -methyl- d -aspartate receptor NR2B subunit in vitro , but its role in vivo is unknown. To clarify this role, we generated transgenic mice overexpressing KIF17 tagged with GFP. The KIF17 transgenic mice exhibited enhanced learning and memory in a series of behavioral tasks, up-regulated NR2B expression with the potential involvement of a transcriptional factor, the cAMP-dependent response element-binding protein, and increased phosphorylation of the cAMP-dependent response element-binding protein. Our results suggest that the motor protein KIF17 contributes to neuronal events required for learning and memory by trafficking fundamental N -methyl- d -aspartate-type glutamate receptors.

Bibliography

Wong, R. W.-C., Setou, M., Teng, J., Takei, Y., & Hirokawa, N. (2002). Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice. Proceedings of the National Academy of Sciences, 99(22), 14500â14505.

Authors 5

Richard Wing-Chuen Wong (first)
Mitsutoshi Setou (additional)
Junlin Teng (additional)
Yosuke Takei (additional)
Nobutaka Hirokawa (additional)

References 57 Referenced 156

Dates

Type	When
Created	22 years, 10 months ago (Oct. 29, 2002, 12:24 p.m.)
Deposited	3 years, 4 months ago (April 25, 2022, 9:08 p.m.)
Indexed	1 day, 1 hour ago (Sept. 3, 2025, 6:09 a.m.)
Issued	22 years, 10 months ago (Oct. 21, 2002)
Published	22 years, 10 months ago (Oct. 21, 2002)
Published Online	22 years, 10 months ago (Oct. 21, 2002)
Published Print	22 years, 10 months ago (Oct. 29, 2002)

Funders 0

None

BibTeX

@article{Wong_2002, title={Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.222371099}, DOI={10.1073/pnas.222371099}, number={22}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wong, Richard Wing-Chuen and Setou, Mitsutoshi and Teng, Junlin and Takei, Yosuke and Hirokawa, Nobutaka}, year={2002}, month=oct, pages={14500–14505} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        3
      ]
    ],
    "date-time": "2025-09-03T10:09:45Z",
    "timestamp": 1756894185989
  },
  "reference-count": 57,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "22",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2002,
        10,
        29
      ]
    ]
  },
  "abstract": "<jats:p>\n            The kinesin superfamily proteins (KIFs) play essential roles in receptor transportation along the microtubules. KIF17 transports the\n            <jats:italic>N</jats:italic>\n            -methyl-\n            <jats:sc>d</jats:sc>\n            -aspartate receptor NR2B subunit\n            <jats:italic>in vitro</jats:italic>\n            , but its role\n            <jats:italic>in vivo</jats:italic>\n            is unknown. To clarify this role, we generated transgenic mice overexpressing KIF17 tagged with GFP. The KIF17 transgenic mice exhibited enhanced learning and memory in a series of behavioral tasks, up-regulated NR2B expression with the potential involvement of a transcriptional factor, the cAMP-dependent response element-binding protein, and increased phosphorylation of the cAMP-dependent response element-binding protein. Our results suggest that the motor protein KIF17 contributes to neuronal events required for learning and memory by trafficking fundamental\n            <jats:italic>N</jats:italic>\n            -methyl-\n            <jats:sc>d</jats:sc>\n            -aspartate-type glutamate receptors.\n          </jats:p>",
  "DOI": "10.1073/pnas.222371099",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        10,
        29
      ]
    ],
    "date-time": "2002-10-29T17:24:11Z",
    "timestamp": 1035912251000
  },
  "page": "14500-14505",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 156,
  "title": "Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice",
  "prefix": "10.1073",
  "volume": "99",
  "author": [
    {
      "given": "Richard Wing-Chuen",
      "family": "Wong",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan"
        }
      ]
    },
    {
      "given": "Mitsutoshi",
      "family": "Setou",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan"
        }
      ]
    },
    {
      "given": "Junlin",
      "family": "Teng",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan"
        }
      ]
    },
    {
      "given": "Yosuke",
      "family": "Takei",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan"
        }
      ]
    },
    {
      "given": "Nobutaka",
      "family": "Hirokawa",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        2002,
        10,
        21
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_3_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.279.5350.519"
    },
    {
      "key": "e_1_3_3_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0955-0674(98)80087-2"
    },
    {
      "key": "e_1_3_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.119.5.1287"
    },
    {
      "key": "e_1_3_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.94.18.9654"
    },
    {
      "key": "e_1_3_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.111145398"
    },
    {
      "key": "e_1_3_3_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.288.5472.1796"
    },
    {
      "key": "e_1_3_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81736-5"
    },
    {
      "key": "e_1_3_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.50.31459"
    },
    {
      "key": "e_1_3_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1329206"
    },
    {
      "key": "e_1_3_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.256.5060.1217"
    },
    {
      "key": "e_1_3_3_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1067020"
    },
    {
      "key": "e_1_3_3_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/361031a0"
    },
    {
      "key": "e_1_3_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0166-2236(95)80016-U"
    },
    {
      "key": "e_1_3_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(01)00321-X"
    },
    {
      "key": "e_1_3_3_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0962-8924(01)02073-6"
    },
    {
      "key": "e_1_3_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0028-3908(01)00122-8"
    },
    {
      "key": "e_1_3_3_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/43432"
    },
    {
      "key": "e_1_3_3_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.274.5293.1678"
    },
    {
      "key": "e_1_3_3_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/29.7.e39"
    },
    {
      "key": "e_1_3_3_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.18-11-04177.1998"
    },
    {
      "key": "e_1_3_3_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1385/MB:15:2:155"
    },
    {
      "key": "e_1_3_3_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M001965200"
    },
    {
      "key": "e_1_3_3_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1385/MB:15:1:65"
    },
    {
      "key": "e_1_3_3_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M004189200"
    },
    {
      "key": "e_1_3_3_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(01)00497-4"
    },
    {
      "key": "e_1_3_3_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/11.5.1475"
    },
    {
      "key": "e_1_3_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(01)00585-2"
    },
    {
      "key": "e_1_3_3_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/297681a0"
    },
    {
      "key": "e_1_3_3_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35050103"
    },
    {
      "key": "e_1_3_3_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81827-9"
    },
    {
      "key": "e_1_3_3_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0168-9525(99)01846-6"
    },
    {
      "key": "e_1_3_3_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0378-1119(98)00005-5"
    },
    {
      "key": "e_1_3_3_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.94.1.129"
    },
    {
      "key": "e_1_3_3_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90691-0"
    },
    {
      "key": "e_1_3_3_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0962-8924(96)10003-9"
    },
    {
      "key": "e_1_3_3_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.200207163"
    },
    {
      "key": "e_1_3_3_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(85)80099-4"
    },
    {
      "key": "e_1_3_3_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/317073a0"
    },
    {
      "key": "e_1_3_3_39_2",
      "first-page": "380",
      "volume": "41",
      "author": "Olton D. S.",
      "year": "1981",
      "unstructured": "Olton D. S. & Feustle, W. A. (1981) Exp. Brain Res.41,380-389.7215498",
      "journal-title": "Exp. Brain Res."
    },
    {
      "key": "e_1_3_3_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0163-1047(93)90664-4"
    },
    {
      "key": "e_1_3_3_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.20-15-05853.2000"
    },
    {
      "key": "e_1_3_3_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1098-1063(1998)8:3<205::AID-HIPO3>3.0.CO;2-I"
    },
    {
      "key": "e_1_3_3_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0028-3932(79)90042-3"
    },
    {
      "key": "e_1_3_3_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1098/rstb.1997.0136"
    },
    {
      "key": "e_1_3_3_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1098/rstb.2001.0945"
    },
    {
      "key": "e_1_3_3_46_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(02)00686-4"
    },
    {
      "key": "e_1_3_3_47_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0959-4388(96)80082-1"
    },
    {
      "key": "e_1_3_3_48_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.neuro.21.1.127"
    },
    {
      "key": "e_1_3_3_49_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0165-0173(97)00050-7"
    },
    {
      "key": "e_1_3_3_50_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s000180050314"
    },
    {
      "key": "e_1_3_3_51_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nn819"
    },
    {
      "key": "e_1_3_3_52_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(02)00684-0"
    },
    {
      "key": "e_1_3_3_53_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(02)00657-8"
    },
    {
      "key": "e_1_3_3_54_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/stke.2001.82.pe1"
    },
    {
      "key": "e_1_3_3_55_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35085068"
    },
    {
      "key": "e_1_3_3_56_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0074-7696(01)04005-0"
    },
    {
      "key": "e_1_3_3_57_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0959-4388(00)00129-X"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.222371099",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        26
      ]
    ],
    "date-time": "2022-04-26T01:08:25Z",
    "timestamp": 1650935305000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.222371099"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2002,
        10,
        21
      ]
    ]
  },
  "references-count": 57,
  "journal-issue": {
    "issue": "22",
    "published-print": {
      "date-parts": [
        [
          2002,
          10,
          29
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.222371099"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.222371099",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        2002,
        10,
        21
      ]
    ]
  },
  "assertion": [
    {
      "value": "2002-06-28",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2002-10-21",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}