A voltage-gated ion channel expressed specifically in spermatozoa
10.1073/pnas.221454998
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

Calcium ions play a primary role in the regulation of sperm cell behavior. We report finding a voltage-gated ion channel (CatSper2) that is expressed in male germ cells but not in other cells. The putative channel contains 6 transmembrane segments, making it more similar to the voltage-gated potassium channels, but the ion selectivity pore domain sequence resembles that of a Ca v channel. The mRNA is expressed during the meiotic or postmeiotic stages of spermatogenesis, and the protein is localized to the sperm flagellum, suggesting a role in the regulation of sperm motility. The mRNA for the channel is present in mouse, rat, and human sperm cells, and the gene is found on chromosome 2 E5–F1 in the mouse and 15q13 in the human. Recently, another voltage-gated channel (CatSper) that has features similar to the one reported here was discovered. It also is expressed within the flagellum and is required for normal fertility of mice. However, expression of CatSper2 alone or coexpression with CatSper in cultured cells, or attempts to coimmunoprecipitate the two proteins from germ cells failed to demonstrate that these two unique but similar α-like subunits form either a homo- or heterotetramer. It is possible, therefore, that two independent α subunits, different from other known voltage-gated channels, regulate sperm motility.

Bibliography

Quill, T. A., Ren, D., Clapham, D. E., & Garbers, D. L. (2001). A voltage-gated ion channel expressed specifically in spermatozoa. Proceedings of the National Academy of Sciences, 98(22), 12527–12531.

Authors 4
  1. Timothy A. Quill (first)
  2. Dejian Ren (additional)
  3. David E. Clapham (additional)
  4. David L. Garbers (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 26, 2002, 10:34 a.m.)
Deposited 3 years, 2 months ago (June 7, 2022, 1:24 a.m.)
Indexed 1 day, 4 hours ago (Aug. 31, 2025, 7:17 p.m.)
Issued 23 years, 10 months ago (Oct. 23, 2001)
Published 23 years, 10 months ago (Oct. 23, 2001)
Published Online 23 years, 10 months ago (Oct. 23, 2001)
Published Print 23 years, 10 months ago (Oct. 23, 2001)
Funders 0

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@article{Quill_2001, title={A voltage-gated ion channel expressed specifically in spermatozoa}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.221454998}, DOI={10.1073/pnas.221454998}, number={22}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Quill, Timothy A. and Ren, Dejian and Clapham, David E. and Garbers, David L.}, year={2001}, month=oct, pages={12527–12531} }