DOI: 10.1073/pnas.221352898. Activation of the Akt-related cytokine-independent survival kinase requires interaction of its phox domain with endosomal phosphatidylinositol 3-phosphate

Activation of the Akt-related cytokine-independent survival kinase requires interaction of its phox domain with endosomal phosphatidylinositol 3-phosphate

10.1073/pnas.221352898

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Protein kinases of the Akt and related serum- and glucocorticoid-regulated kinase (SGK) families are major downstream mediators of phosphatidylinositol (PI) 3-kinase signaling to many cellular processes including metabolic flux, membrane trafficking, and apoptosis. Activation of these kinases is thought to occur at the plasma membrane through their serine and threonine phosphorylation by the phosphoinositide-dependent kinase 1 (PDK1) protein kinase, which interacts with membrane 3′-polyphosphoinositides through its pleckstrin homology (PH) domain. Here, we demonstrate that the SGK family member cytokine-independent survival kinase (CISK) binds strongly and selectively to the monophosphoinositide PI(3)P through its phox homology (PX) domain. Comparing native green fluorescent protein-CISK (EGFP-CISK) to a mutant EGFP-CISK (Y51A) that displays attenuated binding to PI(3)P reveals that this interaction is both necessary and sufficient for its localization to early endosome antigen (EEA1)-positive endosomes. Furthermore, early endosome association of expressed epitope-tagged CISK in COS cells directed by binding of its PX domain to PI(3)P is required for activation of the CISK protein kinase by both insulin-like growth factor-1 and epidermal growth factor. Taken together, these results reveal a critical role of endosomal PI(3)P in the signal transmission mechanism whereby this survival kinase is activated in response to PI3-kinase stimulation by growth factors.

Bibliography

Virbasius, J. V., Song, X., Pomerleau, D. P., Zhan, Y., Zhou, G. W., & Czech, M. P. (2001). Activation of the Akt-related cytokine-independent survival kinase requires interaction of its phox domain with endosomal phosphatidylinositol 3-phosphate. Proceedings of the National Academy of Sciences, 98(23), 12908â12913.

Authors 6

Joseph V. Virbasius (first)
Xi Song (additional)
Darcy P. Pomerleau (additional)
Yong Zhan (additional)
G. Wayne Zhou (additional)
Michael P. Czech (additional)

References 43 Referenced 74

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 8:34 a.m.)
Indexed	1 year ago (Aug. 6, 2024, 6:17 p.m.)
Issued	23 years, 10 months ago (Oct. 23, 2001)
Published	23 years, 10 months ago (Oct. 23, 2001)
Published Online	23 years, 10 months ago (Oct. 23, 2001)
Published Print	23 years, 9 months ago (Nov. 6, 2001)

Funders 0

None

BibTeX

@article{Virbasius_2001, title={Activation of the Akt-related cytokine-independent survival kinase requires interaction of its phox domain with endosomal phosphatidylinositol 3-phosphate}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.221352898}, DOI={10.1073/pnas.221352898}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Virbasius, Joseph V. and Song, Xi and Pomerleau, Darcy P. and Zhan, Yong and Zhou, G. Wayne and Czech, Michael P.}, year={2001}, month=oct, pages={12908–12913} }

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