DOI: 10.1073/pnas.212483099. Higher order arrangement of the eukaryotic nuclear bodies

Higher order arrangement of the eukaryotic nuclear bodies

10.1073/pnas.212483099

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The nuclei of eukaryotic cells consist of discrete substructures. These substructures include the nuclear bodies, which have been implicated in a number of biological processes such as transcription and splicing. However, for most nuclear bodies, the details of involvement in these processes in relation to their three-dimensional distributions in the nucleus are still unclear. Through the analysis of TDP, a protein functional in both transcriptional repression and alternative splicing, we have identified a new category of nuclear bodies within which the TDP molecules reside. Remarkably, the TDP bodies (TBs) colocalize or overlap with several different types of nuclear bodies previously suggested to function in transcription or splicing. Of these nuclear bodies, the Gemini of coiled bodies (GEM) seems to associate with TB through the interaction between survival motor neuron (SMN) protein and TDP. Furthermore, TB sometimes appears to be the bridge of two or more of these other nuclear bodies. Our data suggest the existence of a hierarchy and possibly functional arrangement of the nuclear bodies within the eukaryotic nuclei.

Bibliography

Wang, I.-F., Reddy, N. M., & Shen, C.-K. J. (2002). Higher order arrangement of the eukaryotic nuclear bodies. Proceedings of the National Academy of Sciences, 99(21), 13583â13588.

Authors 3

I-Fan Wang (first)
Narsa M. Reddy (additional)
C.-K. James Shen (additional)

References 37 Referenced 161

10.1126/science.280.5363.547
10.1016/S0955-0674(98)80007-0
10.1016/S0962-8924(99)01606-2
10.1126/science.291.5505.843
10.1038/35066075
10.1016/S0092-8674(02)00626-8
10.1002/j.1460-2075.1996.tb00725.x
10.1073/pnas.97.19.10330
10.1242/jcs.113.22.4043
10.1016/S0960-9822(01)00632-7
10.1093/emboj/18.15.4308
10.1128/jvi.69.6.3584-3596.1995
10.1093/emboj/20.7.1774
10.1146/annurev.bi.62.070193.001445
10.1074/jbc.272.35.22227
10.1074/jbc.M104236200
J Sambrook, E F Fritsch, T Maniatis Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. Press, 2nd Ed., Plainview, NY, 1989). / Molecular Cloning: A Laboratory Manual by Sambrook J (1989)
E Harlow, D Lane Antibodies (Cold Spring Harbor Lab. Press, Plainview, NY, 1998). / Antibodies by Harlow E (1998)
10.1128/MCB.20.19.7401-7409.2000
10.1002/j.1460-2075.1991.tb07712.x
10.1016/S0092-8674(00)81632-3
10.1084/jem.173.6.1407
10.1016/0092-8674(95)90460-3
10.1093/hmg/9.13.1977
10.1101/gad.908401
10.1073/pnas.95.9.4991
10.1093/emboj/19.22.6185
10.1038/343437a0
10.1083/jcb.129.2.287
10.1093/emboj/16.6.1401
10.1006/geno.1999.5746
10.1083/jcb.151.7.1561
10.1006/excr.2000.5097
10.1146/annurev.cb.09.110193.001405
10.1126/science.8446901
10.1016/S0074-7696(08)61231-0
10.1002/j.1460-2075.1996.tb00649.x

Dates

Type	When
Created	22 years, 10 months ago (Oct. 15, 2002, 12:43 p.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 8:10 a.m.)
Indexed	1 month ago (July 23, 2025, 8:55 a.m.)
Issued	22 years, 10 months ago (Oct. 2, 2002)
Published	22 years, 10 months ago (Oct. 2, 2002)
Published Online	22 years, 10 months ago (Oct. 2, 2002)
Published Print	22 years, 10 months ago (Oct. 15, 2002)

Funders 0

None

BibTeX

@article{Wang_2002, title={Higher order arrangement of the eukaryotic nuclear bodies}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.212483099}, DOI={10.1073/pnas.212483099}, number={21}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wang, I-Fan and Reddy, Narsa M. and Shen, C.-K. James}, year={2002}, month=oct, pages={13583–13588} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        23
      ]
    ],
    "date-time": "2025-07-23T12:55:02Z",
    "timestamp": 1753275302360
  },
  "reference-count": 37,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "21",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2002,
        10,
        15
      ]
    ]
  },
  "abstract": "<jats:p>The nuclei of eukaryotic cells consist of discrete substructures. These substructures include the nuclear bodies, which have been implicated in a number of biological processes such as transcription and splicing. However, for most nuclear bodies, the details of involvement in these processes in relation to their three-dimensional distributions in the nucleus are still unclear. Through the analysis of TDP, a protein functional in both transcriptional repression and alternative splicing, we have identified a new category of nuclear bodies within which the TDP molecules reside. Remarkably, the TDP bodies (TBs) colocalize or overlap with several different types of nuclear bodies previously suggested to function in transcription or splicing. Of these nuclear bodies, the Gemini of coiled bodies (GEM) seems to associate with TB through the interaction between survival motor neuron (SMN) protein and TDP. Furthermore, TB sometimes appears to be the bridge of two or more of these other nuclear bodies. Our data suggest the existence of a hierarchy and possibly functional arrangement of the nuclear bodies within the eukaryotic nuclei.</jats:p>",
  "DOI": "10.1073/pnas.212483099",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        10,
        15
      ]
    ],
    "date-time": "2002-10-15T16:43:03Z",
    "timestamp": 1034700183000
  },
  "page": "13583-13588",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 161,
  "title": "Higher order arrangement of the eukaryotic nuclear bodies",
  "prefix": "10.1073",
  "volume": "99",
  "author": [
    {
      "given": "I-Fan",
      "family": "Wang",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Institute of Molecular Biology, Academia Sinica, and Institute of Molecular Medicine, National Taiwan University, Taipei, Taiwan 115, Republic of China"
        }
      ]
    },
    {
      "given": "Narsa M.",
      "family": "Reddy",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Institute of Molecular Biology, Academia Sinica, and Institute of Molecular Medicine, National Taiwan University, Taipei, Taiwan 115, Republic of China"
        }
      ]
    },
    {
      "given": "C.-K. James",
      "family": "Shen",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Institute of Molecular Biology, Academia Sinica, and Institute of Molecular Medicine, National Taiwan University, Taipei, Taiwan 115, Republic of China"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        2002,
        10,
        2
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_3_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.280.5363.547"
    },
    {
      "key": "e_1_3_3_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0955-0674(98)80007-0"
    },
    {
      "key": "e_1_3_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0962-8924(99)01606-2"
    },
    {
      "key": "e_1_3_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.291.5505.843"
    },
    {
      "key": "e_1_3_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35066075"
    },
    {
      "key": "e_1_3_3_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(02)00626-8"
    },
    {
      "key": "e_1_3_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1996.tb00725.x"
    },
    {
      "key": "e_1_3_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.97.19.10330"
    },
    {
      "key": "e_1_3_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.113.22.4043"
    },
    {
      "key": "e_1_3_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0960-9822(01)00632-7"
    },
    {
      "key": "e_1_3_3_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/18.15.4308"
    },
    {
      "key": "e_1_3_3_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.69.6.3584-3596.1995"
    },
    {
      "key": "e_1_3_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/20.7.1774"
    },
    {
      "key": "e_1_3_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.62.070193.001445"
    },
    {
      "key": "e_1_3_3_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.35.22227"
    },
    {
      "key": "e_1_3_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M104236200"
    },
    {
      "key": "e_1_3_3_17_2",
      "volume-title": "Molecular Cloning: A Laboratory Manual",
      "author": "Sambrook J",
      "year": "1989",
      "unstructured": "J Sambrook, E F Fritsch, T Maniatis Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. Press, 2nd Ed., Plainview, NY, 1989).",
      "edition": "2"
    },
    {
      "key": "e_1_3_3_18_2",
      "volume-title": "Antibodies",
      "author": "Harlow E",
      "year": "1998",
      "unstructured": "E Harlow, D Lane Antibodies (Cold Spring Harbor Lab. Press, Plainview, NY, 1998)."
    },
    {
      "key": "e_1_3_3_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.20.19.7401-7409.2000"
    },
    {
      "key": "e_1_3_3_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1991.tb07712.x"
    },
    {
      "key": "e_1_3_3_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81632-3"
    },
    {
      "key": "e_1_3_3_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1084/jem.173.6.1407"
    },
    {
      "key": "e_1_3_3_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90460-3"
    },
    {
      "key": "e_1_3_3_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/hmg/9.13.1977"
    },
    {
      "key": "e_1_3_3_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.908401"
    },
    {
      "key": "e_1_3_3_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.95.9.4991"
    },
    {
      "key": "e_1_3_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/19.22.6185"
    },
    {
      "key": "e_1_3_3_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/343437a0"
    },
    {
      "key": "e_1_3_3_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.129.2.287"
    },
    {
      "key": "e_1_3_3_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/16.6.1401"
    },
    {
      "key": "e_1_3_3_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/geno.1999.5746"
    },
    {
      "key": "e_1_3_3_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.151.7.1561"
    },
    {
      "key": "e_1_3_3_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/excr.2000.5097"
    },
    {
      "key": "e_1_3_3_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.cb.09.110193.001405"
    },
    {
      "key": "e_1_3_3_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8446901"
    },
    {
      "key": "e_1_3_3_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0074-7696(08)61231-0"
    },
    {
      "key": "e_1_3_3_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1996.tb00649.x"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.212483099",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T12:10:55Z",
    "timestamp": 1649851855000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.212483099"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2002,
        10,
        2
      ]
    ]
  },
  "references-count": 37,
  "journal-issue": {
    "issue": "21",
    "published-print": {
      "date-parts": [
        [
          2002,
          10,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.212483099"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.212483099",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        2002,
        10,
        2
      ]
    ]
  },
  "assertion": [
    {
      "value": "2002-03-11",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2002-08-12",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2002-10-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}