DOI: 10.1073/pnas.211400898. A high-speed atomic force microscope for studying biological macromolecules

A high-speed atomic force microscope for studying biological macromolecules

10.1073/pnas.211400898

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The atomic force microscope (AFM) is a powerful tool for imaging individual biological molecules attached to a substrate and placed in aqueous solution. At present, however, it is limited by the speed at which it can successively record highly resolved images. We sought to increase markedly the scan speed of the AFM, so that in the future it can be used to study the dynamic behavior of biomolecules. For this purpose, we have developed a high-speed scanner, free of resonant vibrations up to 60 kHz, small cantilevers with high resonance frequencies (450–650 kHz) and small spring constants (150–280 pN/nm), an objective-lens type of deflection detection device, and several electronic devices of wide bandwidth. Integration of these various devices has produced an AFM that can capture a 100 × 100 pixel 2 image within 80 ms and therefore can generate a movie consisting of many successive images (80-ms intervals) of a sample in aqueous solution. This is demonstrated by imaging myosin V molecules moving on mica (see http://www.s.kanazawa-u.ac.jp/phys/biophys/bmv_movie.htm ).

Bibliography

Ando, T., Kodera, N., Takai, E., Maruyama, D., Saito, K., & Toda, A. (2001). A high-speed atomic force microscope for studying biological macromolecules. Proceedings of the National Academy of Sciences, 98(22), 12468â12472.

Authors 6

Toshio Ando (first)
Noriyuki Kodera (additional)
Eisuke Takai (additional)
Daisuke Maruyama (additional)
Kiwamu Saito (additional)
Akitoshi Toda (additional)

References 21 Referenced 970

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:34 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 8:57 a.m.)
Indexed	30 minutes ago (Aug. 29, 2025, 9:29 a.m.)
Issued	23 years, 10 months ago (Oct. 9, 2001)
Published	23 years, 10 months ago (Oct. 9, 2001)
Published Online	23 years, 10 months ago (Oct. 9, 2001)
Published Print	23 years, 10 months ago (Oct. 23, 2001)

Funders 0

None

BibTeX

@article{Ando_2001, title={A high-speed atomic force microscope for studying biological macromolecules}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.211400898}, DOI={10.1073/pnas.211400898}, number={22}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Ando, Toshio and Kodera, Noriyuki and Takai, Eisuke and Maruyama, Daisuke and Saito, Kiwamu and Toda, Akitoshi}, year={2001}, month=oct, pages={12468–12472} }

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