DOI: 10.1073/pnas.211266898. Visualization of protein S1 within the 30S ribosomal subunit and its interaction with messenger RNA

Visualization of protein S1 within the 30S ribosomal subunit and its interaction with messenger RNA

10.1073/pnas.211266898

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

S1 is the largest ribosomal protein, present in the small subunit of the bacterial ribosome. It has a pivotal role in stabilizing the mRNA on the ribosome. Thus far, S1 has eluded structural determination. We have identified the S1 protein mass in the cryo-electron microscopic map of theEscherichia coliribosome by comparing the map with a recent x-ray crystallographic structure of the 30S subunit, which lacks S1. According to our finding, S1 is located at the junction of head, platform, and main body of the 30S subunit, thus explaining all existing biochemical and crosslinking data. Protein S1 as identified in our map has a complex, elongated shape with two holes in its central portion. The N-terminal domain, forming one of the extensions, penetrates into the head of the 30S subunit. Evidence for direct interaction of S1 with 11 nucleotides of the mRNA, immediately upstream of the Shine–Dalgarno sequence, explains the protein's role in the recognition of the 5′ region of mRNA.

Bibliography

Sengupta, J., Agrawal, R. K., & Frank, J. (2001). Visualization of protein S1 within the 30S ribosomal subunit and its interaction with messenger RNA. Proceedings of the National Academy of Sciences, 98(21), 11991â11996.

Authors 3

Jayati Sengupta (first)
Rajendra K. Agrawal (additional)
Joachim Frank (additional)

References 54 Referenced 129

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U Bommer, N Burkhardt, R Jünemann, C M T Spahn, F J Triana-Alonso, K H Nierhaus Subcellular Fractionation: A Practical Approach, eds J Graham, D Rickwood (IRL, Washington, DC), pp. 271–301 (1997). (10.1093/oso/9780199634958.003.0009) / Subcellular Fractionation: A Practical Approach by Bommer U (1997)
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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:34 a.m.)
Deposited	1 year, 7 months ago (Jan. 3, 2024, 11:49 p.m.)
Indexed	3 months, 3 weeks ago (April 30, 2025, 12:09 a.m.)
Issued	23 years, 10 months ago (Oct. 2, 2001)
Published	23 years, 10 months ago (Oct. 2, 2001)
Published Online	23 years, 10 months ago (Oct. 2, 2001)
Published Print	23 years, 10 months ago (Oct. 9, 2001)

Funders 0

None

BibTeX

@article{Sengupta_2001, title={Visualization of protein S1 within the 30S ribosomal subunit and its interaction with messenger RNA}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.211266898}, DOI={10.1073/pnas.211266898}, number={21}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sengupta, Jayati and Agrawal, Rajendra K. and Frank, Joachim}, year={2001}, month=oct, pages={11991–11996} }

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