DOI: 10.1073/pnas.2036346100. Association of the Mediator complex with enhancers of active genes

Association of the Mediator complex with enhancers of active genes

10.1073/pnas.2036346100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The multiprotein Mediator complex has been shown to interact with gene-specific regulatory proteins and RNA polymerase II in vitro . Here, we use chromatin immunoprecipitation to analyze the recruitment of Mediator to GAL genes of yeast in vivo . We find that Mediator associates exclusively with transcriptionally active and not inactive GAL genes. This association maps to the upstream activating sequence, rather than the core promoter, and is independent of RNA polymerase II, general transcription factors, and core promoter sequences. These findings support the idea of Mediator as a primary conduit of regulatory information from enhancers to promoters in eukaryotic cells.

Bibliography

Kuras, L., Borggrefe, T., & Kornberg, R. D. (2003). Association of the Mediator complex with enhancers of active genes. Proceedings of the National Academy of Sciences, 100(24), 13887â13891.

Authors 3

Laurent Kuras (first)
Tilman Borggrefe (additional)
Roger D. Kornberg (additional)

References 34 Referenced 87

10.1016/0092-8674(90)90683-6
10.1016/0092-8674(81)90019-2
10.1016/0092-8674(90)90685-8
10.1038/350436a0
10.1016/0092-8674(94)90221-6
10.1038/383188a0
10.1038/383185a0
10.1126/science.288.5469.1244
10.1126/science.288.5469.1242
10.1093/emboj/cdg336
10.1146/annurev.cellbio.13.1.1
10.1146/annurev.genet.34.1.77
10.1146/annurev.biochem.69.1.729
10.1016/S0092-8674(00)81641-4
10.1128/mr.51.4.458-476.1987
10.1074/jbc.M109581200
10.1038/21239
10.1016/0092-8674(92)90040-J
10.1073/pnas.93.11.5208
10.1073/pnas.92.24.10864
Bash, R. & Lohr, D. (2001) Prog. Nucleic Acid Res. Mol. Biol. 65, 197–259.11008489 / Prog. Nucleic Acid Res. Mol. Biol. (2001)
10.1038/21232
10.1126/science.283.5404.985
10.1021/bi010011k
10.1016/S1097-2765(02)00598-1
10.1038/386569a0
10.1016/0092-8674(93)90362-T
10.1073/pnas.94.12.6075
10.1016/S1097-2765(03)00144-8
10.1101/gad.921601
10.1016/S1097-2765(01)00266-0
10.1016/S1097-2765(01)00296-9
10.1073/pnas.93.16.8329
10.1016/S0968-0004(00)01596-6

Dates

Type	When
Created	21 years, 9 months ago (Nov. 25, 2003, 3:59 p.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 7:29 a.m.)
Indexed	1 year ago (Aug. 4, 2024, 5:15 p.m.)
Issued	21 years, 9 months ago (Nov. 17, 2003)
Published	21 years, 9 months ago (Nov. 17, 2003)
Published Online	21 years, 9 months ago (Nov. 17, 2003)
Published Print	21 years, 9 months ago (Nov. 25, 2003)

Funders 0

None

BibTeX

@article{Kuras_2003, title={Association of the Mediator complex with enhancers of active genes}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.2036346100}, DOI={10.1073/pnas.2036346100}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kuras, Laurent and Borggrefe, Tilman and Kornberg, Roger D.}, year={2003}, month=nov, pages={13887–13891} }

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