DOI: 10.1073/pnas.202604399. Parallel phenotypic analysis of sporulation and postgermination growth in <i>Saccharomyces</i> <i>cerevisiae</i>

Parallel phenotypic analysis of sporulation and postgermination growth in Saccharomyces cerevisiae

10.1073/pnas.202604399

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have quantitatively monitored the sporulation and germination efficiencies of ≈4,200 yeast deletion strains in parallel by using a molecular bar coding strategy. In a single study, we doubled the number of genes functionally implicated in sporulation to ≈400, identifying both positive and negative regulators. Our set of 261 sporulation-deficient genes illustrates the importance of autophagy, carbon utilization, and transcriptional machinery during sporulation. These general cellular factors are more likely to exhibit fitness defects when deleted and less likely to be transcriptionally regulated than sporulation-specific genes. Our postgermination screening assay identified recombination/chromosome segregation genes, aneuploid strains, and possible germination-specific factors. Finally, our results facilitate a genome-wide comparison of expression pattern and mutant phenotype for a developmental process and suggest that 16% of genes differentially expressed during sporulation confer altered efficiency of spore production or defective postgermination growth when disrupted.

Bibliography

Deutschbauer, A. M., Williams, R. M., Chu, A. M., & Davis, R. W. (2002). Parallel phenotypic analysis of sporulation and postgermination growth in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences, 99(24), 15530â15535.

Authors 4

Adam M. Deutschbauer (first)
Roy M. Williams (additional)
Angela M. Chu (additional)
Ronald W. Davis (additional)

References 28 Referenced 152

10.1093/nar/25.1.28
10.1093/genetics/61.1.79
10.1016/S0960-9822(01)00274-3
10.1002/yea.843
10.1038/319738a0
10.1126/science.282.5389.699
10.1038/82539
10.1038/nature00935
10.1038/ng1296-450
10.1073/pnas.231366398
10.1126/science.1065672
10.1038/ng929
10.1002/(SICI)1097-0061(19980130)14:2<115::AID-YEA204>3.0.CO;2-2
10.1073/pnas.92.14.6479
10.1016/0076-6879(91)94012-2
Burke D. Dawson D. & Stearns T. (2000) Methods in Yeast Genetics (Cold Spring Harbor Lab. Press Plainview NY).
10.1126/science.285.5429.901
10.1083/jcb.119.2.301
10.1128/aem.61.2.630-638.1995
10.1093/emboj/18.2.320
10.1128/MCB.20.22.8364-8372.2000
10.1016/S0070-2153(01)52008-6
10.1016/S0092-8674(00)00217-8
10.1038/77116
10.1016/S0021-9258(19)36578-0
10.1016/0167-4838(94)00201-Q
10.1073/pnas.92.26.12490
10.1093/genetics/153.3.1131

Dates

Type	When
Created	22 years, 9 months ago (Nov. 26, 2002, 12:42 p.m.)
Deposited	3 years, 4 months ago (April 25, 2022, 8:56 p.m.)
Indexed	4 days, 16 hours ago (Aug. 28, 2025, 8:19 a.m.)
Issued	22 years, 9 months ago (Nov. 13, 2002)
Published	22 years, 9 months ago (Nov. 13, 2002)
Published Online	22 years, 9 months ago (Nov. 13, 2002)
Published Print	22 years, 9 months ago (Nov. 26, 2002)

Funders 0

None

BibTeX

@article{Deutschbauer_2002, title={Parallel phenotypic analysis of sporulation and postgermination growth in Saccharomyces cerevisiae}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.202604399}, DOI={10.1073/pnas.202604399}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Deutschbauer, Adam M. and Williams, Roy M. and Chu, Angela M. and Davis, Ronald W.}, year={2002}, month=nov, pages={15530–15535} }

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