DOI: 10.1073/pnas.1932773100. Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells

Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells

10.1073/pnas.1932773100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

This report describes an unbiased method for systematically determining gene function in mammalian cells. A total of 20,704 predicted human full-length cDNAs were tested for induction of the IL-8 promoter. A number of genes, including those for cytokines, receptors, adapters, kinases, and transcription factors, were identified that induced the IL-8 promoter through known regulatory sites. Proteins that acted through a cooperative interaction between an AP-1 and an unrecognized cAMP response element (CRE)-like site were also identified. A protein, termed transducer of regulated cAMP response element-binding protein (CREB) (TORC1), was identified that activated expression through the variant CRE and consensus CRE sites. TORC1 potently induced known CREB1 target genes, bound CREB1, and activated expression through a potent transcription activation domain. A functionalDrosophilaTORC gene was also identified. Thus, TORCs represent a family of highly conserved CREB coactivators that may control the potency and specificity of CRE-mediated responses.

Bibliography

Iourgenko, V., Zhang, W., Mickanin, C., Daly, I., Jiang, C., Hexham, J. M., Orth, A. P., Miraglia, L., Meltzer, J., Garza, D., Chirn, G.-W., McWhinnie, E., Cohen, D., Skelton, J., Terry, R., Yu, Y., Bodian, D., Buxton, F. P., Zhu, J., â¦ Labow, M. A. (2003). Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells. Proceedings of the National Academy of Sciences, 100(21), 12147â12152.

Authors 21

Vadim Iourgenko (first)
Wenjun Zhang (additional)
Craig Mickanin (additional)
Ira Daly (additional)
Can Jiang (additional)
Jonathan M. Hexham (additional)
Anthony P. Orth (additional)
Loren Miraglia (additional)
Jodi Meltzer (additional)
Dan Garza (additional)
Gung-Wei Chirn (additional)
Elizabeth McWhinnie (additional)
Dalia Cohen (additional)
Joanne Skelton (additional)
Robert Terry (additional)
Yang Yu (additional)
Dale Bodian (additional)
Frank P. Buxton (additional)
Jian Zhu (additional)
Chuanzheng Song (additional)
Mark A. Labow (additional)

References 29 Referenced 329

10.1038/35057062
10.1126/science.1058040
10.1038/nature01262
10.1038/nature01266
10.1073/pnas.242603899
10.1016/S0168-9525(02)00005-7
10.1089/107999099313866
Hoffmann, E., Dittrich-Breiholz, O., Holtmann, H. & Kracht, M. (2002) J. Leukocyte Biol. 72, 847–855.12429706 (10.1189/jlb.72.5.847) / J. Leukocyte Biol. (2002)
10.1074/jbc.M202941200
10.1002/1521-4141(200208)32:8<2208::AID-IMMU2208>3.0.CO;2-2
10.1074/jbc.272.4.2396
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10.1007/s100380050183
10.1182/blood.V87.11.4607.bloodjournal87114607
10.1006/cyto.2000.0759
10.1093/nar/22.8.1463
10.1038/ng1083
Walton, K. M., Rehfuss, R. P., Chrivia, J. C., Lochner, J. E. & Goodman, R. H. (1992) Mol. Endocrinol. 6, 647–655.1350057 / Mol. Endocrinol. (1992)
10.1016/S0021-9258(19)81599-5
10.1016/S0021-9258(18)55177-2
10.1128/MCB.15.2.653
Bianco, C., Tortora, G., Baldassarre, G., Caputo, R., Fontanini, G., Chine, S., Bianco, A. R. & Ciardiello, F. (1997) Clin. Cancer Res. 3, 439–448.9815703 / Clin. Cancer Res. (1997)
10.1038/365314a0
10.1111/j.1432-1033.1996.00393.x
10.1016/j.molcel.2003.08.013
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10.1111/j.1476-5381.1996.tb16000.x
10.1016/0140-6736(93)92695-P
10.1038/nbt795

Dates

Type	When
Created	21 years, 10 months ago (Oct. 14, 2003, 3:05 p.m.)
Deposited	2 years, 4 months ago (April 27, 2023, 12:52 a.m.)
Indexed	5 days, 20 hours ago (Aug. 30, 2025, 12:52 p.m.)
Issued	21 years, 11 months ago (Sept. 23, 2003)
Published	21 years, 11 months ago (Sept. 23, 2003)
Published Online	21 years, 11 months ago (Sept. 23, 2003)
Published Print	21 years, 10 months ago (Oct. 14, 2003)

Funders 0

None

BibTeX

@article{Iourgenko_2003, title={Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1932773100}, DOI={10.1073/pnas.1932773100}, number={21}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Iourgenko, Vadim and Zhang, Wenjun and Mickanin, Craig and Daly, Ira and Jiang, Can and Hexham, Jonathan M. and Orth, Anthony P. and Miraglia, Loren and Meltzer, Jodi and Garza, Dan and Chirn, Gung-Wei and McWhinnie, Elizabeth and Cohen, Dalia and Skelton, Joanne and Terry, Robert and Yu, Yang and Bodian, Dale and Buxton, Frank P. and Zhu, Jian and Song, Chuanzheng and Labow, Mark A.}, year={2003}, month=sep, pages={12147–12152} }

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