DOI: 10.1073/pnas.1835776100. Unifying features in protein-folding mechanisms

Unifying features in protein-folding mechanisms

10.1073/pnas.1835776100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We compare the folding of representative members of a protein superfamily by experiment and simulation to investigate common features in folding mechanisms. The homeodomain superfamily of three-helical, single-domain proteins exhibits a spectrum of folding processes that spans the complete transition from concurrent secondary and tertiary structure formation (nucleation-condensation mechanism) to sequential secondary and tertiary formation (framework mechanism). The unifying factor in their mechanisms is that the transition state for (un)folding is expanded and very native-like, with the proportion and degree of formation of secondary and tertiary interactions varying. There is a transition, or slide, from the framework to nucleation-condensation mechanism with decreasing stability of the secondary structure. Thus, framework and nucleation-condensation are different manifestations of an underlying common mechanism.

Bibliography

Gianni, S., Guydosh, N. R., Khan, F., Caldas, T. D., Mayor, U., White, G. W. N., DeMarco, M. L., Daggett, V., & Fersht, A. R. (2003). Unifying features in protein-folding mechanisms. Proceedings of the National Academy of Sciences, 100(23), 13286â13291.

Authors 9

Stefano Gianni (first)
Nicholas R. Guydosh (additional)
Faaizah Khan (additional)
Teresa D. Caldas (additional)
Ugo Mayor (additional)
George W. N. White (additional)
Mari L. DeMarco (additional)
Valerie Daggett (additional)
Alan R. Fersht (additional)

References 45 Referenced 217

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Dates

Type	When
Created	21 years, 9 months ago (Nov. 16, 2003, 3:42 p.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:57 a.m.)
Indexed	1 month ago (July 30, 2025, 10:26 a.m.)
Issued	21 years, 10 months ago (Oct. 31, 2003)
Published	21 years, 10 months ago (Oct. 31, 2003)
Published Online	21 years, 10 months ago (Oct. 31, 2003)
Published Print	21 years, 9 months ago (Nov. 11, 2003)

Funders 0

None

BibTeX

@article{Gianni_2003, title={Unifying features in protein-folding mechanisms}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1835776100}, DOI={10.1073/pnas.1835776100}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Gianni, Stefano and Guydosh, Nicholas R. and Khan, Faaizah and Caldas, Teresa D. and Mayor, Ugo and White, George W. N. and DeMarco, Mari L. and Daggett, Valerie and Fersht, Alan R.}, year={2003}, month=oct, pages={13286–13291} }

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