DOI: 10.1073/pnas.1835685100. Endogenous neosynthesis vs. cross-presentation of viral antigens for cytotoxic T cell priming

Endogenous neosynthesis vs. cross-presentation of viral antigens for cytotoxic T cell priming

10.1073/pnas.1835685100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Induction of antiviral cytotoxic T lymphocytes (CTLs) has been proposed to require cross-presentation of viral antigens derived from infected extralymphatic host cells by antigen-presenting cells (APC). This postulated mechanism of cross-priming is thought to be essential for CTL responses against viruses that do not infect professional APC, e.g., because of absence of the specific virus receptor. Here, we show for the human pathogen poliovirus that naturally nonpermissive murine APC acquire viral RNAin vivoindependently of the cellular virus receptor. Uptake of poliovirus or polioviral RNA initiated neosynthesis of viral antigen to an extent sufficient to prime CTLsin vivo, which were detectable 2-3 wk after infection. Our results do not only indicate that experiments studying cross-presentation and cross-priming by using potentially amplifiable or translatable materials need careful examination, but they also question the general biological importance of cross-presentation and cross-priming in antiviral CTL responses.

Bibliography

Freigang, S., Egger, D., Bienz, K., Hengartner, H., & Zinkernagel, R. M. (2003). Endogenous neosynthesis vs. cross-presentation of viral antigens for cytotoxic T cell priming. Proceedings of the National Academy of Sciences, 100(23), 13477â13482.

Authors 5

Stefan Freigang (first)
Denise Egger (additional)
Kurt Bienz (additional)
Hans Hengartner (additional)
Rolf M. Zinkernagel (additional)

References 38 Referenced 48

Dates

Type	When
Created	21 years, 9 months ago (Nov. 16, 2003, 3:42 p.m.)
Deposited	2 years, 4 months ago (April 27, 2023, 9:31 a.m.)
Indexed	4 months, 4 weeks ago (March 30, 2025, 7:03 p.m.)
Issued	21 years, 9 months ago (Oct. 31, 2003)
Published	21 years, 9 months ago (Oct. 31, 2003)
Published Online	21 years, 9 months ago (Oct. 31, 2003)
Published Print	21 years, 9 months ago (Nov. 11, 2003)

Funders 0

None

BibTeX

@article{Freigang_2003, title={Endogenous neosynthesis vs. cross-presentation of viral antigens for cytotoxic T cell priming}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1835685100}, DOI={10.1073/pnas.1835685100}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Freigang, Stefan and Egger, Denise and Bienz, Kurt and Hengartner, Hans and Zinkernagel, Rolf M.}, year={2003}, month=oct, pages={13477–13482} }

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