DOI: 10.1073/pnas.182021199. SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription

SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription

10.1073/pnas.182021199

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Spt-Ada-Gcn5 acetyltransferase (SAGA) is a previously described histone acetyltransferase/transcriptional coactivator complex in yeast. At promoters of certain genes ( HIS3 and TRP3 ), SAGA has an inhibitory function involving a nonproductive TATA-binding protein interaction mediated by the Spt3 and Spt8 subunits. Related to this, Spt8-less SAGA is a major form of the complex under activating conditions for these genes. In the present study, we purify this activation-specific complex, called SALSA (SAGA altered, Spt8 absent). Besides lacking Spt8, SALSA contains Spt7 subunit that is truncated. Examining the role of this subunit, we find that C-terminally truncated SPT7 resulted in derepressed HIS3 transcription. Furthermore, when grown in rich media (repressing conditions), wild-type cells yielded predominantly SAGA, but Spt7 C-terminal truncations resulted primarily in a form of complex similar to SALSA. Thus, SALSA-like structure and activating function can be partially recapitulated in yeast by truncating the C terminus of Spt7. Overall, these results lead to a model that for a subset of promoters SAGA is inhibitory through Spt3, Spt8, and an Spt8-interacting subdomain of Spt7, whereas SALSA is a form of complex for positive transcriptional regulation. These data clarify a mechanism by which a transcriptional regulatory complex can switch between positive and negative modulation.

Bibliography

Sterner, D. E., Belotserkovskaya, R., & Berger, S. L. (2002). SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription. Proceedings of the National Academy of Sciences, 99(18), 11622â11627.

Authors 3

David E. Sterner (first)
Rimma Belotserkovskaya (additional)
Shelley L. Berger (additional)

References 43 Referenced 99

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Dates

Type	When
Created	22 years, 11 months ago (Sept. 3, 2002, 3:58 p.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:11 a.m.)
Indexed	1 year, 2 months ago (June 13, 2024, 7:16 p.m.)
Issued	23 years ago (Aug. 19, 2002)
Published	23 years ago (Aug. 19, 2002)
Published Online	23 years ago (Aug. 19, 2002)
Published Print	22 years, 11 months ago (Sept. 3, 2002)

Funders 0

None

BibTeX

@article{Sterner_2002, title={SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.182021199}, DOI={10.1073/pnas.182021199}, number={18}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sterner, David E. and Belotserkovskaya, Rimma and Berger, Shelley L.}, year={2002}, month=aug, pages={11622–11627} }

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