DOI: 10.1073/pnas.181354198. Self-organization of a radial microtubule array by dynein-dependent nucleation of microtubules

Self-organization of a radial microtubule array by dynein-dependent nucleation of microtubules

10.1073/pnas.181354198

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Polarized radial arrays of cytoplasmic microtubules (MTs) with minus ends clustered at the cell center define the organization of the cytoplasm through interaction with microtubule motors bound to membrane organelles or chromosomes. It is generally assumed that the radial organization results from nucleation of MTs at the centrosome. However, radial MT array can also be attained through self-organization that requires the activity of a minus-end-directed MT motor, cytoplasmic dynein. In this study we examine the role of cytoplasmic dynein in the self-organization of a radial MT array in cytoplasmic fragments of fish melanophores lacking the centrosome. After activation of dynein motors bound to membrane-bound organelles, pigment granules, the fragments rapidly form polarized radial arrays of MTs and position pigment aggregates at their centers. We show that rearrangement of MTs in the cytoplasm is achieved through dynein-dependent MT nucleation. The radial pattern is generated by continuous disassembly and reassembly of MTs and concurrent minus-end-directed transport of pigment granules bearing the nucleation sites.

Bibliography

Vorobjev, I., Malikov, V., & Rodionov, V. (2001). Self-organization of a radial microtubule array by dynein-dependent nucleation of microtubules. Proceedings of the National Academy of Sciences, 98(18), 10160â10165.

Authors 3

Ivan Vorobjev (first)
Viacheslav Malikov (additional)
Vladimir Rodionov (additional)

References 46 Referenced 72

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:37 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:15 a.m.)
Indexed	1 year ago (Aug. 4, 2024, 2:35 p.m.)
Issued	24 years ago (Aug. 14, 2001)
Published	24 years ago (Aug. 14, 2001)
Published Online	24 years ago (Aug. 14, 2001)
Published Print	23 years, 11 months ago (Aug. 28, 2001)

Funders 0

None

BibTeX

@article{Vorobjev_2001, title={Self-organization of a radial microtubule array by dynein-dependent nucleation of microtubules}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.181354198}, DOI={10.1073/pnas.181354198}, number={18}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Vorobjev, Ivan and Malikov, Viacheslav and Rodionov, Vladimir}, year={2001}, month=aug, pages={10160–10165} }

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