DOI: 10.1073/pnas.181338798. Self-organization of a propulsive actin network as an evolutionary process

Self-organization of a propulsive actin network as an evolutionary process

10.1073/pnas.181338798

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The leading edge of motile cells is propelled by polymerization of actin filaments according to a dendritic nucleation/array treadmilling mechanism. However, little attention has been given to the origin and maintenance of the dendritic array. Here we develop and test a population–kinetics model that explains the organization of actin filaments in terms of the reproduction of dendritic units. The life cycle of an actin filament consists of dendritic nucleation on another filament (birth), elongation by addition of actin subunits and, finally, termination of filament growth by capping protein (death). The regularity of branch angle between daughter and mother filaments endows filaments with heredity of their orientation. Fluctuations of branch angle that become fixed in the actin network create errors of orientation (mutations) that may be inherited. In our model, birth and death rates depend on filament orientation, which then becomes a selectable trait. Differential reproduction and elimination of filaments, or natural selection, leads to the evolution of a filament pattern with a characteristic distribution of filament orientations. We develop a procedure based on the Radon transform for quantitatively analyzing actin networksin situand show that the experimental results are in agreement with the distribution of filament orientations predicted by our model. We conclude that the propulsive actin network can be understood as a self-organizing supramolecular ensemble shaped by the evolution of dendritic lineages through natural selection of their orientation.

Bibliography

Maly, I. V., & Borisy, G. G. (2001). Self-organization of a propulsive actin network as an evolutionary process. Proceedings of the National Academy of Sciences, 98(20), 11324â11329.

Authors 2

Ivan V. Maly (first)
Gary G. Borisy (additional)

References 29 Referenced 119

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:34 a.m.)
Deposited	1 year, 7 months ago (Jan. 3, 2024, 11:48 p.m.)
Indexed	2 months, 3 weeks ago (June 5, 2025, 6:48 a.m.)
Issued	23 years, 11 months ago (Sept. 25, 2001)
Published	23 years, 11 months ago (Sept. 25, 2001)
Published Online	23 years, 11 months ago (Sept. 25, 2001)
Published Print	23 years, 11 months ago (Sept. 25, 2001)

Funders 0

None

BibTeX

@article{Maly_2001, title={Self-organization of a propulsive actin network as an evolutionary process}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.181338798}, DOI={10.1073/pnas.181338798}, number={20}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Maly, Ivan V. and Borisy, Gary G.}, year={2001}, month=sep, pages={11324–11329} }

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