DOI: 10.1073/pnas.180293097. Unbinding process of adsorbed proteins under external stress studied by atomic force microscopy spectroscopy

Unbinding process of adsorbed proteins under external stress studied by atomic force microscopy spectroscopy

10.1073/pnas.180293097

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We report the study of the dynamics of the unbinding process under a force load f of adsorbed proteins (fibrinogen) on a solid surface (hydrophilic silica) by means of atomic force microscopy spectroscopy. By varying the loading rate r f , defined by f = r f t, t being the time, we find that, as for specific interactions, the mean rupture force increases with r f . This unbinding process is analyzed in the framework of the widely used Bell model. The typical dissociation rate at zero force entering in the model lies between 0.02 and 0.6 s −1 . Each measured rupture is characterized by a force f 0 , which appears to be quantized in integer multiples of 180–200 pN.

Bibliography

Gergely, C., Voegel, J.-C., Schaaf, P., Senger, B., Maaloum, M., HÃ¶rber, J. K. H., & HemmerlÃ©, J. (2000). Unbinding process of adsorbed proteins under external stress studied by atomic force microscopy spectroscopy. Proceedings of the National Academy of Sciences, 97(20), 10802â10807.

Authors 7

C. Gergely (first)
J.-C. Voegel (additional)
P. Schaaf (additional)
B. Senger (additional)
M. Maaloum (additional)
J. K. H. Hörber (additional)
J. Hemmerlé (additional)

References 20 Referenced 58

10.1021/la981052t
10.1073/pnas.96.12.6705
10.1126/science.7939660
10.1016/S0006-3495(96)79814-4
10.1073/pnas.95.21.12283
10.1016/S0006-3495(98)77666-0
10.1038/16219
10.1021/la981003g
10.1021/la990259z
10.1006/jmbi.1998.2466
10.1016/S0968-0004(99)01453-X
10.1006/jcis.1997.5247
10.1006/cbir.1997.0215
10.1126/science.347575
10.1016/S0006-3495(97)78802-7
10.1088/0034-4885/62/6/202
M Abramowitz, I A Stegun Handbook of Mathematical Functions (Dover, New York, 1970). / Handbook of Mathematical Functions by Abramowitz M (1970)
10.1073/pnas.96.20.11277
10.1016/0956-5663(95)99227-C
10.1016/0001-8686(86)80012-4

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:41 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 4:07 a.m.)
Indexed	1 year, 10 months ago (Oct. 9, 2023, 2:47 p.m.)
Issued	24 years, 11 months ago (Sept. 12, 2000)
Published	24 years, 11 months ago (Sept. 12, 2000)
Published Online	24 years, 11 months ago (Sept. 12, 2000)
Published Print	24 years, 11 months ago (Sept. 26, 2000)

Funders 0

None

BibTeX

@article{Gergely_2000, title={Unbinding process of adsorbed proteins under external stress studied by atomic force microscopy spectroscopy}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.180293097}, DOI={10.1073/pnas.180293097}, number={20}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Gergely, C. and Voegel, J.-C. and Schaaf, P. and Senger, B. and Maaloum, M. and Hörber, J. K. H. and Hemmerlé, J.}, year={2000}, month=sep, pages={10802–10807} }

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