DOI: 10.1073/pnas.1716305114. Proteasomes tether to two distinct sites at the nuclear pore complex

Proteasomes tether to two distinct sites at the nuclear pore complex

10.1073/pnas.1716305114

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Significance This study compares the native structures of cytosolic and nuclear proteasomes, visualized directly within cells. The assembly states and functional states of proteasomes in each compartment were similar, indicating comparable levels of proteolytic activity per proteasome. Nuclear proteasomes were tethered to two different sites at the nuclear pore complex (NPC): the inner nuclear membrane and the NPC basket. Structural analysis revealed mechanistic details of the two tethering interactions. These results present direct evidence that proteasomes bind at NPCs, establishing a cellular hub for protein degradation at the gateway between the nucleus and cytoplasm. This work demonstrates how cryo-electron tomography can reveal biological mechanisms by directly observing the interactions between molecular complexes within the native cellular environment.

Bibliography

Albert, S., Schaffer, M., Beck, F., Mosalaganti, S., Asano, S., Thomas, H. F., Plitzko, J. M., Beck, M., Baumeister, W., & Engel, B. D. (2017). Proteasomes tether to two distinct sites at the nuclear pore complex. Proceedings of the National Academy of Sciences, 114(52), 13726â13731.

Authors 10

Sahradha Albert (first)
Miroslava Schaffer (additional)
Florian Beck (additional)
Shyamal Mosalaganti (additional)
Shoh Asano (additional)
Henry F. Thomas (additional)
Jürgen M. Plitzko (additional)
Martin Beck (additional)
Wolfgang Baumeister (additional)
Benjamin D. Engel (additional)

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Dates

Type	When
Created	7 years, 8 months ago (Dec. 11, 2017, 3:05 p.m.)
Deposited	3 years, 2 months ago (June 7, 2022, 12:53 p.m.)
Indexed	1 week, 6 days ago (Aug. 7, 2025, 5:05 p.m.)
Issued	7 years, 8 months ago (Dec. 11, 2017)
Published	7 years, 8 months ago (Dec. 11, 2017)
Published Online	7 years, 8 months ago (Dec. 11, 2017)
Published Print	7 years, 7 months ago (Dec. 26, 2017)

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Deutsche Forschungsgemeinschaft 10.13039/501100001659
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Deutsche Forschungsgemeinschaft 10.13039/501100001659
Region: Europe
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1. German Research Association
2. German Research Foundation
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Awards1
1. SFB-1035 / Project A01
EC | FP7 | FP7 Ideas: European Research Council 10.13039/100011199 FP7 Ideas: European Research Council
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1. FP7-IDEAS-ERC - Specific Programme: 'Ideas' Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013)
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BibTeX

@article{Albert_2017, title={Proteasomes tether to two distinct sites at the nuclear pore complex}, volume={114}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1716305114}, DOI={10.1073/pnas.1716305114}, number={52}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Albert, Sahradha and Schaffer, Miroslava and Beck, Florian and Mosalaganti, Shyamal and Asano, Shoh and Thomas, Henry F. and Plitzko, Jürgen M. and Beck, Martin and Baumeister, Wolfgang and Engel, Benjamin D.}, year={2017}, month=dec, pages={13726–13731} }

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