DOI: 10.1073/pnas.1618206114. High-numerical-aperture cryogenic light microscopy for increased precision of superresolution reconstructions

High-numerical-aperture cryogenic light microscopy for increased precision of superresolution reconstructions

10.1073/pnas.1618206114

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Significance The precision of localization-based superresolution microscopy techniques fundamentally relies on the point-spread function of the optical system and the number of photons one can collect. Here, we report that by using a high-numerical-aperture objective lens and a custom cryogenic stage, we are able to increase photon yield by 2–3-fold over room temperature, thereby achieving more precise superresolution reconstructions of complex subcellular structures.

Bibliography

Nahmani, M., Lanahan, C., DeRosier, D., & Turrigiano, G. G. (2017). High-numerical-aperture cryogenic light microscopy for increased precision of superresolution reconstructions. Proceedings of the National Academy of Sciences, 114(15), 3832â3836.

Authors 4

Marc Nahmani (first)
Conor Lanahan (additional)
David DeRosier (additional)
Gina G. Turrigiano (additional)

References 18 Referenced 37

Dates

Type	When
Created	8 years, 5 months ago (March 27, 2017, 9 p.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:22 a.m.)
Indexed	1 week, 1 day ago (Aug. 23, 2025, 9:27 p.m.)
Issued	8 years, 5 months ago (March 27, 2017)
Published	8 years, 5 months ago (March 27, 2017)
Published Online	8 years, 5 months ago (March 27, 2017)
Published Print	8 years, 4 months ago (April 11, 2017)

Funders 2

HHS | NIH | NIH Office of the Director 10.13039/100000052 NIH Office of the Director
Region: Americas
gov (National government)
Labels4
1. Office of the Director
2. Office of the NIH Director
3. Office of the Director, NIH
4. OD
Awards1
1. DP1 003388
HHS | NIH | National Institute of Neurological Disorders and Stroke 10.13039/100000065 National Institute of Neurological Disorders and Stroke
Region: Americas
gov (National government)
Labels10
1. National Institute of Neurological Disorders & Stroke
2. NIH/National Institute of Neurological Disorders and Stroke
3. NIH National Institute of Neurological Disorders and Stroke
4. Instituto Nacional de Trastornos Neurológicos y Accidentes Cerebrovasculares
5. The National Institute of Neurological Disorders and Stroke
6. National Institute of Neurological Disorders and Blindness
7. National Institute of Neurological and Communicative Disorders and Stroke
8. NINDS
9. NINDB
10. NINCDS
Awards1
1. R37 NS092635

BibTeX

@article{Nahmani_2017, title={High-numerical-aperture cryogenic light microscopy for increased precision of superresolution reconstructions}, volume={114}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1618206114}, DOI={10.1073/pnas.1618206114}, number={15}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Nahmani, Marc and Lanahan, Conor and DeRosier, David and Turrigiano, Gina G.}, year={2017}, month=mar, pages={3832–3836} }

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