DOI: 10.1073/pnas.1614614113. Structural basis for dynamic regulation of the human 26S proteasome

Structural basis for dynamic regulation of the human 26S proteasome

10.1073/pnas.1614614113

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Significance The proteasome holoenzyme is an ATP-dependent protease in eukaryotes that degrades ubiquitylated substrates. It is involved in numerous important biological processes, such as cell division, differentiation, innate immunity, adaptive immunity, regulation of gene expression, and response to proteotoxic stress. Using cryoelectron microscopy, we have examined multiple conformational states of the human proteasome at medium to high resolution. Our results reveal that the substrate-conducting channel in the core particle is transiently opened and accompanied by dynamic changes in structure of the particle. These observations provide new insights into how the proteasome recognizes ubiquitylated substrates and translocates them through a channel and gate to degradation sites in the core particle.

Bibliography

Chen, S., Wu, J., Lu, Y., Ma, Y.-B., Lee, B.-H., Yu, Z., Ouyang, Q., Finley, D. J., Kirschner, M. W., & Mao, Y. (2016). Structural basis for dynamic regulation of the human 26S proteasome. Proceedings of the National Academy of Sciences, 113(46), 12991â12996.

Authors 10

Shuobing Chen (first)
Jiayi Wu (additional)
Ying Lu (additional)
Yong-Bei Ma (additional)
Byung-Hoon Lee (additional)
Zhou Yu (additional)
Qi Ouyang (additional)
Daniel J. Finley (additional)
Marc W. Kirschner (additional)
Youdong Mao (additional)

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Dates

Type	When
Created	8 years, 10 months ago (Oct. 21, 2016, 11:40 p.m.)
Deposited	3 years, 2 months ago (June 7, 2022, 11:03 a.m.)
Indexed	1 month ago (July 27, 2025, 3:51 a.m.)
Issued	8 years, 10 months ago (Oct. 21, 2016)
Published	8 years, 10 months ago (Oct. 21, 2016)
Published Online	8 years, 10 months ago (Oct. 21, 2016)
Published Print	8 years, 9 months ago (Nov. 15, 2016)

Funders 3

HHS | NIH | National Institute of General Medical Sciences 10.13039/100000057 National Institute of General Medical Sciences
Region: Americas
gov (National government)
Labels2
1. U.S. National Institute of General Medical Sciences
2. NIGMS
Awards1
1. GM026875
National Natural Science Foundation of China 10.13039/501100001809
Region: Asia
gov (National government)
Labels11
1. Chinese National Science Foundation
2. Natural Science Foundation of China
3. National Science Foundation of China
4. NNSF of China
5. NSF of China
6. 国家自然科学基金委员会
7. National Nature Science Foundation of China
8. Guójiā Zìrán Kēxué Jījīn Wěiyuánhuì
9. NSFC
10. NNSF
11. NNSFC
Awards1
1. 91530321
HHS | NIH | National Institute of General Medical Sciences 10.13039/100000057 National Institute of General Medical Sciences
Region: Americas
gov (National government)
Labels2
1. U.S. National Institute of General Medical Sciences
2. NIGMS
Awards1
1. GM43601

BibTeX

@article{Chen_2016, title={Structural basis for dynamic regulation of the human 26S proteasome}, volume={113}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1614614113}, DOI={10.1073/pnas.1614614113}, number={46}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Chen, Shuobing and Wu, Jiayi and Lu, Ying and Ma, Yong-Bei and Lee, Byung-Hoon and Yu, Zhou and Ouyang, Qi and Finley, Daniel J. and Kirschner, Marc W. and Mao, Youdong}, year={2016}, month=oct, pages={12991–12996} }

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