DOI: 10.1073/pnas.1534635100. Single metallic nanoparticle imaging for protein detection in cells

Single metallic nanoparticle imaging for protein detection in cells

10.1073/pnas.1534635100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We performed a visualization of membrane proteins labeled with 10-nm gold nanoparticles in cells, using an all-optical method based on photothermal interference contrast. The high sensitivity of the method and the stability of the signals allows 3D imaging of individual nanoparticles without the drawbacks of photobleaching and blinking inherent to fluorescent markers. A simple analytical model is derived to account for the measurements of the signal amplitude and the spatial resolution. The photothermal interference contrast method provides an efficient, reproducible, and promising way to visualize low amounts of proteins in cells by optical means.

Bibliography

Cognet, L., Tardin, C., Boyer, D., Choquet, D., Tamarat, P., & Lounis, B. (2003). Single metallic nanoparticle imaging for protein detection in cells. Proceedings of the National Academy of Sciences, 100(20), 11350â11355.

Authors 6

L. Cognet (first)
C. Tardin (additional)
D. Boyer (additional)
D. Choquet (additional)
P. Tamarat (additional)
B. Lounis (additional)

References 42 Referenced 295

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Dates

Type	When
Created	21 years, 10 months ago (Oct. 1, 2003, 3:44 p.m.)
Deposited	3 years, 4 months ago (April 25, 2022, 9:36 p.m.)
Indexed	1 month ago (July 28, 2025, 2:40 a.m.)
Issued	21 years, 11 months ago (Sept. 17, 2003)
Published	21 years, 11 months ago (Sept. 17, 2003)
Published Online	21 years, 11 months ago (Sept. 17, 2003)
Published Print	21 years, 10 months ago (Sept. 30, 2003)

Funders 0

None

BibTeX

@article{Cognet_2003, title={Single metallic nanoparticle imaging for protein detection in cells}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1534635100}, DOI={10.1073/pnas.1534635100}, number={20}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Cognet, L. and Tardin, C. and Boyer, D. and Choquet, D. and Tamarat, P. and Lounis, B.}, year={2003}, month=sep, pages={11350–11355} }

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