In situ structural analysis of Golgi intracisternal protein arrays
10.1073/pnas.1515337112
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

Significance To our knowledge, this is the first detailed study of Golgi ultrastructure within unperturbed cells. Three intracisternal structures were identified, with implications for Golgi architecture and trafficking: ( i ) Bundles of filaments show how cargoes may oligomerize to increase their local concentration at trans-Golgi buds. ( ii ) Granular aggregates provide evidence for cisternal maturation, as they are likely too large to transit the Golgi via vesicles. ( iii ) Protein arrays link the membranes of the central trans-Golgi cisternae, simultaneously maintaining the narrow luminal spacing while promoting cargo exit from the Golgi periphery by excluding material from the center. The asymmetry of the array structure indicates that the apposing membranes of a single cisterna have distinct compositions. The assembly of arrays may also enhance glycosyltransferase kinetics.

Bibliography

Engel, B. D., Schaffer, M., Albert, S., Asano, S., Plitzko, J. M., & Baumeister, W. (2015). In situ structural analysis of Golgi intracisternal protein arrays. Proceedings of the National Academy of Sciences, 112(36), 11264–11269.

Authors 6
  1. Benjamin D. Engel (first)
  2. Miroslava Schaffer (additional)
  3. Sahradha Albert (additional)
  4. Shoh Asano (additional)
  5. Jürgen M. Plitzko (additional)
  6. Wolfgang Baumeister (additional)
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Dates
Type When
Created 9 years, 11 months ago (Aug. 26, 2015, 10:30 p.m.)
Deposited 3 years, 2 months ago (June 7, 2022, 9:27 a.m.)
Indexed 3 weeks, 6 days ago (July 24, 2025, 7:46 a.m.)
Issued 9 years, 11 months ago (Aug. 26, 2015)
Published 9 years, 11 months ago (Aug. 26, 2015)
Published Online 9 years, 11 months ago (Aug. 26, 2015)
Published Print 9 years, 11 months ago (Sept. 8, 2015)
Funders 5
  1. Alexander von Humboldt-Stiftung 10.13039/100005156

    Region: Europe

    pri (Trusts, charities, foundations (both public and private))

    Labels5
    1. Humboldt-Stiftung
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    3. Alexander von Humboldt Foundation
    4. Humboldt Stiftung
    5. AvH
    Awards1
    1. research fellowship
  2. European Commission 10.13039/501100000780

    Region: Europe

    gov (National government)

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    1. European Union
    2. Comisión Europea
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    4. EU-Kommissionen
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    6. Ευρωπαϊκής Επιτροπής
    7. Европейската комисия
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    11. Europskoj komisiji
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    23. Euroopan komission
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    Awards1
    1. HEALTH-F4-2008-201648/PROSPECTS
  3. European Commission 10.13039/501100000780

    Region: Europe

    gov (National government)

    Labels26
    1. European Union
    2. Comisión Europea
    3. Europäische Kommission
    4. EU-Kommissionen
    5. Euroopa Komisjoni
    6. Ευρωπαϊκής Επιτροπής
    7. Европейската комисия
    8. Evropské komise
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    11. Europskoj komisiji
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    13. La Commissione europea
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    Awards1
    1. ERC-2012-SyG_318987-ToPAG
  4. Deutsche Forschungsgemeinschaft 10.13039/501100001659

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    gov (National government)

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    1. CIPSM
  5. Deutsche Forschungsgemeinschaft 10.13039/501100001659

    Region: Europe

    gov (National government)

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    1. German Research Association
    2. German Research Foundation
    3. DFG
    Awards1
    1. SFB 1035

@article{Engel_2015, title={In situ structural analysis of Golgi intracisternal protein arrays}, volume={112}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1515337112}, DOI={10.1073/pnas.1515337112}, number={36}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Engel, Benjamin D. and Schaffer, Miroslava and Albert, Sahradha and Asano, Shoh and Plitzko, Jürgen M. and Baumeister, Wolfgang}, year={2015}, month=aug, pages={11264–11269} }