DOI: 10.1073/pnas.1512783112. ClpB N-terminal domain plays a regulatory role in protein disaggregation

ClpB N-terminal domain plays a regulatory role in protein disaggregation

10.1073/pnas.1512783112

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Significance ClpB/Hsp100 chaperones protect cells from the devastating effects of protein inactivation and aggregation arising from extreme stress. This function is accomplished first by binding to the aggregates and then forcibly unraveling individual proteins by passing them through the central channel in the hexameric chaperones. Here, we investigate the role of the ClpB/Hsp100 N-terminal domain (NTD) in protein disaggregation. Our results demonstrate that ClpB recognizes exposed hydrophobic stretches in unfolded or aggregated client proteins via a substrate-binding groove in its NTD. We further show that the NTD has regulatory roles that include blocking the translocation channel in the absence of substrate and destabilizing client proteins upon binding, thus priming them for subsequent unfolding and disaggregation.

Bibliography

Rosenzweig, R., Farber, P., Velyvis, A., Rennella, E., Latham, M. P., & Kay, L. E. (2015). ClpB N-terminal domain plays a regulatory role in protein disaggregation. Proceedings of the National Academy of Sciences, 112(50).

Authors 6

Rina Rosenzweig (first)
Patrick Farber (additional)
Algirdas Velyvis (additional)
Enrico Rennella (additional)
Michael P. Latham (additional)
Lewis E. Kay (additional)

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Dates

Type	When
Created	9 years, 8 months ago (Nov. 30, 2015, 11:15 p.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:42 a.m.)
Indexed	4 weeks ago (Aug. 2, 2025, 1:26 a.m.)
Issued	9 years, 9 months ago (Nov. 30, 2015)
Published	9 years, 9 months ago (Nov. 30, 2015)
Published Online	9 years, 9 months ago (Nov. 30, 2015)
Published Print	9 years, 8 months ago (Dec. 15, 2015)

Funders 2

Gouvernement du Canada | Canadian Institutes of Health Research 10.13039/501100000024 Canadian Institutes of Health Research
Region: Americas
gov (National government)
Labels6
1. Instituts de Recherche en Santé du Canada
2. Canadian Institutes of Health Research (CIHR)
3. CIHR_IRSC
4. Canadian Institutes of Health Research | Ottawa ON
5. CIHR
6. IRSC
Awards1
1. 404101
Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada 10.13039/501100000038 Natural Sciences and Engineering Research Council of Canada
Region: Americas
gov (National government)
Labels3
1. Conseil de Recherches en Sciences Naturelles et en Génie du Canada
2. NSERC
3. CRSNG
Awards1
1. 455908

BibTeX

@article{Rosenzweig_2015, title={ClpB N-terminal domain plays a regulatory role in protein disaggregation}, volume={112}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.1512783112}, DOI={10.1073/pnas.1512783112}, number={50}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Rosenzweig, Rina and Farber, Patrick and Velyvis, Algirdas and Rennella, Enrico and Latham, Michael P. and Kay, Lewis E.}, year={2015}, month=nov }

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